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Preparation of Novel Porous Magnetic Microspheres and Application in Immobilized Enzyme Field

Author: ZengLiXi
Tutor: DengLe
School: Hunan Normal University
Course: Microbiology
Keywords: Magnetic polymer composite microspheres Immobilized enzyme Papain
CLC: Q814
Type: Master's thesis
Year: 2006
Downloads: 423
Quote: 2
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Abstract


The immobilized enzyme can improve the stability of the enzyme and increase the frequency of use of the enzyme as well as ease of separation of product and substrate are widely depth studies . The core problem of the immobilized enzyme technology is focused on the preparation of the carrier material and selection , as well as to improve the nature of the immobilized enzyme , etc. . The bioactive polymer magnetic materials is an important mutual penetration of Polymer Science and Life Sciences generated the edge of the field , many unique advantages as an immobilized enzyme carrier . The hydrophilic glycidyl methacrylate as the monomer, ethylene glycol dimethacrylate ester as the crosslinking agent , through improved suspension polymerization in the presence of a porogen will After Surface Modification Fe 3 O 4 magnetic nanoparticles entrapped in which the synthesis of a high hydrophilic , uniform particle size , dispersion , and surface functional epoxy group - rich magnetic porous polymer composite microspheres . Papain as a model enzyme, immobilized enzyme study by covalent bonding method . Best immobilized papain , prepared under the optimized conditions of high activity , the good performance of the immobilized enzyme and its catalytic activity compared with the free enzyme are not significantly impact . Casein as the substrate , enzymatic properties and kinetic parameters of the immobilized enzyme . The results show that : the immobilized enzyme optimum reaction temperature of 80 ℃, the optimum reaction pH of 7.5 , the K m values ??4.25mg/mL enzyme activity recovery was 55.2 % . Immobilized enzyme thermal stability , pH stability and operational stability than the free enzyme has been greatly improved . Further magnetic porous polymer composite microspheres amination modified with supple the hydrophilic chain dialdehyde starch (DAS) and then combined with the enzyme immobilized enzyme , found that the unit amount of immobilized enzyme decreased slightly , enzyme the live recovery rate , the Michaelis constant (K m ) than the free enzyme decreases and improve the affinity of the enzyme substrate . Through research, this new type of magnetic porous polymer composite microspheres can be used as excellent Immobilized Enzymes that this magnetic microspheres immobilized enzyme and other related biomedical field will have a broad application prospects .

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CLC: > Biological Sciences > Bioengineering ( Biotechnology ) > Enzyme Engineering
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