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Application of Chromatographic Techniques in the Seperation and Analysis of Active Ingredients in Medicinal Plants of Scutellaria Baicalensis Etc.

Author: YangHongMei
Tutor: ZengJianGuo；ChenBo
School: Hunan Normal University
Course: Analytical Chemistry
Keywords: High Performance Liquid Chromatography Preparative liquid chromatography Macroporous resin Scutellaria Oroxylum Americas pepper Polygonum cuspidatum
CLC: R284
Type: Master's thesis
Year: 2006
Downloads: 261
Quote: 0
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Abstract


Purification and analysis of traditional Chinese medicine active ingredient is an important part of the modernization of Chinese medicine . For a long time , the quality control of TCM lack of clear indicators , the standard reference substance and effective way , a great influence on Chinese medicine products to the international market . Of this thesis on the problems in the quality control of Chinese medicine , such as : macroporous resin and preparative high performance liquid chromatography and high performance liquid chromatography - mass spectrometry with activity in several Chinese medicine with modern chromatographic separation , purification and analysis techniques components purification and analysis of research . The thesis research work are as follows : 1 to the preparation magnolia alkali and Hengzhou aggregata base standard reference substance for the goal, the macroporous resin and preparative high performance liquid chromatography separation the Mulan alkali and Hengzhou in the the purification Americas pepper Ukraine drugs alkali . Macroporous resin and preparative high performance liquid chromatography separation conditions . The method for the first time using a macroporous resin were prepared products desalination and the short production cycle , high yield and purity of the product can reach more than 95% of the advantages . 2 . Establish cuspidatum extract resveratrol , emodin , chrysophanol , emodin four active ingredients content was measured by high performance liquid chromatography . The method can be used to cuspidatum herbs and their extracts quality control . 3 using high-performance liquid chromatography , diode array detector and electrospray ionization mass spectrometry analysis skullcap skullcap extract flavonoids , and other than the standard method of one of the eight components of quantitative analysis , to establish a more comprehensive skullcap extract quality control methods . First application of high performance liquid chromatography and diode array detector and electrospray ionization mass spectrometry

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CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Traditional Chinese medicine chemical
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