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The Effects of C-myc Antisense Oligodeoxynucleotide on Apoptosis, Proliferation and Cell Cycle of Hepatocellular Carcinoma Cells

Author: LiZhenJun
Tutor: GuoTianKang；LiYiPing；SuHe
School: Lanzhou University
Course: Surgery
Keywords: C-myc antisense oligodeoxynucleotide hepatocellular carcinoma cell
CLC: R735.7
Type: Master's thesis
Year: 2006
Downloads: 58
Quote: 0
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Abstract


Objectives To investigate the effect of C-myc antisense oligo-deoxynucleotide （ASODN） on growth ,apoptosis and cell cycle of HEPG-2 cells.Methods HEPG-2 cells were cultured conventionally. Growth inhibition rate of HEPG-2 cells ,after having been treated with C-myc ASODN, were detected by MTT method. Morphological change of cells were observed under a fluorescent microscope. DNA ladder was examined by DNA eiectrophoresis. The apoptotic rate and cell cycle phase distribution were examined by flow cytometer analysis. The expression of C-myc gene was detected by immunohistochemistry staining and RT-PCR on the mRNA or protein level respectively.Results The result of immunohistochemical and RT-PCR assays showedthat C-myc ASODN could decrease the expression of C-myc gene . The growth inhibition of HEPG-2 cells, after having been treated with C-myc ASODN, was certained by MTT analysis. Furthermore ,with the concentration increasing of C-myc ASODN and time passing, the inhibition ability also elevated. Cell apoptosis was identified by fluorescent staining, DNA eiectrophoresis and flow cytometer. At the same time ,C-myc ASODN could block cells cycle on G0/G1 phase by flow cytometer analysis.Conclusions （1） C-myc ASODN could inhibit the growth of HEPG-2 cells.（2） C-myc ASODN could induce apoptosis of HEPG-2 cells. （3）C-myc ASODN could block cells cycle on G0/G1 phase.

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Liver tumors
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