Dissertation > Excellent graduate degree dissertation topics show

The Study of Association between Apolipoprotein C3 Gene Polymorphism and Hypertensive Disorder Complicating Pregnancy

Author: ZhaoRuiFen
Tutor: ZhangWeiYuan
School: Jilin University
Course: Clinical
Keywords: Hypertensive disorders in pregnancy Apolipoprotein C3 Gene polymorphism
CLC: R714.246
Type: Master's thesis
Year: 2006
Downloads: 82
Quote: 0
Read: Download Dissertation


Hypertensive disorder complicating pregnancy (HDCP) is acharacteristic disease during pregnancy period. It is one of thediseases that seriously threatened maternal and perinatal safety andis still one of the main causes of maternal and perinatal mortality sofar. There were many pathogenetic hypotheses about hypertensivedisorder complicating pregnancy, but it is not clear yet. so it is veryimportant to study the etiology in order to prevent and cure HDCP.Literatures reported that lipid metabolic disorder wasassociation with HDCP. Genetic factor was proofed contributing tothe risk of HDCP. And the SstI polymorphism of the apo C3 gene isassociated with HTG in many races. Considered these three reasonwe designed this study to reveal association betweenapolipoprotein C3 gene polymorphism and hypertensive disordercomplicating pregnancy. Methods: In our study, 84 patients with HDCP and 81 normalcontrols were recruited. They were all single birth, primiparity andmatched for age. DNA was extracted from peripheral blood.Genotyping was performed by using PCR-based restrictionfragment length polymorphism (RFLP) method. The products ofenzyme digestion were separated by electrophoresis in 2.5%agarose gel, dyed with EB and analysed by gel aerial image system.Datum were analysed with SPSS software.Results: 1.The genotypic distribution was in agreement withHardy-Weinberg equilibrium。2.The frequencies of Apo C3 SstⅠS1S1, S2S1, S2S2 genotypes were 48.81%, 36.90% and 14.29%respectively in HDCP group, and in control group were 54.32%,35.80% and 9.87% respectively. Between control group and HDCPgroup the frequencies distribution of genotypes was no statisticallysignificant difference ( χ 2=0.9075 P>0.05). 3.The frequencies ofthe two alleles(S1、S2)were 72.22% and 27.78% respectively inHDCP group, and were 67.06% and 32.94% respectively in controlgroup.Prevalence of S2 allele in normal controls and HDCPpatients were comparable(P>0.05). 3. There was no statisticaldifference in genotypic distribution between HDCP1 complicgroup and HDCP2 complic group. And there was no statisticallysignificant in allele frequencies comparing gestational hypertensionsubset, mild-pre-eclampsia subset, severe-pre-eclampsia subset,and eclampsia subset. 4.There was no statistical difference in thesystolic pressure of different Apo C3 SstⅠgenotypes(P>0.05), aswell as in the diastolic pressure (P>0.05). The difference in thepositive rate of proteinuria of the different Apo C3 SstⅠgenotypeswas no statistically significant(P>0.05). 5.The results of relativerisk analysis of Apo C3 SstⅠgenotypes distribution and alleleswere,S2S2/S1S1:OR=1.6098(95%CI:0.5960~4.3484),S2S2/S2S1:OR=1.4032(95%CI:0.5013~3.9280),S2S1/S1S1:OR=1.1842(95%CI:0.5362~2.6155),S2+/S2-:OR=1.2472(95%CI:0.9674~1.6080). Genotypes S2S2 and S2S1 didn’tincrease the risk of HDCP in our study, or S2 allele did neither.Conclusion: Our study didn’t show a significant associationbetween Apo C3 gene SstⅠ polymorphism and HDCP. And ApoC3 gene SstⅠ polymorphism wasn’t associated with the severityof HDCP either in our study. Genotypes S2S2 and S2S1 didn’tincrease the risk of HDCP in our study, or S2 allele did neither.We failed to show an association between the S2 allele of apoC-III and HDCP. The lack of association between HDCP and apoC-III SstⅠ polymorphism may be a result of the limited number ofpatients included in our study, or the fact that we studid only onepolymorphism of apo C-III gene, which had more than 5polymorphsim as we knew. Future larger works will need to clarifywhether the apo C-III polymorphism affects susceptibility toHDCP.

Related Dissertations

  1. CYP2C19 Gene Polymorphism in Patients with Ischemic Cerebrovascular,R743
  2. The Relationship of Polymorphisms of Methionine Synthase Reductase(MTRR) Gene and Plasma Homocysteine Levels with Myocardial Infarction,R542.22
  3. Association of Interleukin-6 Gene Polymorphisms with Susceptibility to Coronary Heart Disease in Asian Populations: a Meta-analysis,R541.4
  4. The Analysis of the Polymorphism of the Plasminogen Activator Urokinase (PLAU) in Alzheimer’s Disease (AD) of Uygurs and Hans in Xinjiang,R749.16
  5. Xinjiang Uygurs and Hans Phosphodiesterase 4D Gene SNP83 Polymorphism Analysis of Ischemic Cerebrovascular Disease,R743.3
  6. Association Study of Sheat Shock Protein 70-2 Gene Single Nucleotide Polymorphism and Diabetic Nephropathy,R587.2
  7. The Relationship Between Tumor Necrosis Factor-βrs1041981 Gene Polymorphisms and Coronary Artery Disease in the Patients of Nanjing Area,R541.4
  8. The Gene Polymorphism and Serum Content of TNF-α in Restenosis Patients after PCI,R541.4
  9. The Associatecl Research between Polymorphism of KIR Gene and the Liver Kidney Yin Insufficiency Type VLS,R275.9
  10. The Association of CTLA-4、 VDR and MIC-A Gene Polymorphism with Latent Autoimmune Diabetes in Adults,R587.1
  11. Association between Estrogen Receptor Gene Polymorphism and Depressive Disorder in Menopausal Women,R749.4
  12. Efficacy Evaluation and Influencing Factors Analysis of Combination Regimens Based on Amlodipine,R544.1
  13. Study on LHβ and GnRHR Gene as the Candidate Gene for Fecundity in Sheep,S826
  14. Study on CYP2C9, VKORC1 Genetic Polymorphisms and Correlation Factors in Warfarin Anticoagulant Therapy Effect,R96
  15. The Study of Assocation of Single Nucleotide Polymorphism of HCR-325 Gene with Psoriasis Vulgaris in the Han Population,R758.63
  16. Study on the Relationship between the Genetic Polymorphism of GSTT1 Gene、Smoking and Lung Cancer Susceptibility,R734.2
  17. Study on Relationship between Adiponectin Gene SNP+45 Polymorphism and Type 2 Diabetes Mellitus Complicated with Carotid IMT,R587.1
  18. Preliminary study the pathogenesis of coronary vasodilator,R543
  19. Studies on IL-10 Gene Promoter -1082 A/G Polymorphism in LADA,R587.1
  20. Associations P27 Gene Polymorphism and Its Protein Production with Endometriosis,R711.71
  21. Studies on Relationship Between Adiponectin Gene Polymorphism and Ischemic Stroke in the Han Chinese Population,R743.3

CLC: > Medicine, health > Obstetrics and Gynaecology > Obstetrics > Pathological pregnancy ( abnormal pregnancy ) > Gestosis > Pregnancy-induced hypertension
© 2012 www.DissertationTopic.Net  Mobile