Dissertation > Excellent graduate degree dissertation topics show

Association between eNOS and ACE Genetic Polymorphisms and the Therapeutic Efficacy of Salvianolate Injection in Coronary Heart Disease Patients

Author: FangChao
Tutor: LiuZhaoQian
School: Central South University
Course: Pharmacy
Keywords: endothelial nitric oxide synthase angiotensin convertingenzyme gene polymorphism coronary heart disease salvianolateinjection
CLC: R96
Type: Master's thesis
Year: 2013
Downloads: 7
Quote: 0
Read: Download Dissertation


Objective:The purpose of this study is to clarify the distribution of eNOS894G/T and ACE I/D genetic polymorphisms in Chinese Han population and to explore the effect of eNOS and ACE gene polymorphism on the response to salvianolate in patients with CHD.Methods:In this study we selected153cases of non-ischemic cardiomyopathy and non-sudden death in patients with coronary heart disease and compared with198healthy controls. We collected5ml peripheral blood to extract DNA. Genetic diagnosis of eNOS894G/T was determined by direct sequencing. Polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP) was used to detect the genotypes in ACE I/D. Clinical characteristics of CHD patients and healthy controls were compared using the Chi-square test. Independent-Sample Test, One-Way ANOVA, Chi-square test and Binary logistic regression were used for statistical analysis of genetic parameters.Results:By genotyping the selected153cases of coronary heart disease patients and198healthy controls for eNOS894G/T and ACE I/D, we observed that,(1) there were significant differences in the frequency distribution of eNOS and ACE polymorphisms between CHD patients and healthy controls (T allele:16.01%vs10.61%, I allele:55.56%vs45.86%; p<0.05).(2) there are significant differences in plasma ApoAl-C between the eNOS G894T genotypes (p<0.05). Meanwhile there are edge statistical differences in plasma TC and LDL cholesterol levels between ACE genotypes(p=0.078and0.063).(3) Binary logistic regression analysis revealed that the genotypes had an additive and dominant effect on patients’therapeutic response (T/T and G/T vs G/G, odds ratio:2.401, p=0.032, I/I vs I/D vs D/D, odds ratio:1.738, p=0.013; T/T and G/T vs G/G, odds ratio:2.446,p=0.028, D/D and I/D vs I/I, odds ratio:2.414, p=0.015)Conclusion:The gene polymorphisms of eNOS gene894G/T and ACE I/D are related to the occurrence of coronary heart disease. These data suggest that genetic variations in eNOS and ACE might affect the response to salvianolate in Chinese CHD patients. Patients carrying eNOS894G or ACE D alleles are good responders to salvianolate therapy.

Related Dissertations

  1. CYP2C19 Gene Polymorphism in Patients with Ischemic Cerebrovascular,R743
  2. The Relationship of Polymorphisms of Methionine Synthase Reductase(MTRR) Gene and Plasma Homocysteine Levels with Myocardial Infarction,R542.22
  3. Association of Interleukin-6 Gene Polymorphisms with Susceptibility to Coronary Heart Disease in Asian Populations: a Meta-analysis,R541.4
  4. Correlation Analysis between Coronary Lesion Severity and Peripheral Blood Cell CD34~+ Ratio in the Coronary Heart Disease Patients Complicating Diabetes Mellitus,R541.4
  5. Study of the Expression of Toll-like Receptor 2, 4mRNA and Polymorphisms of Patients with Leprosy,R755
  6. MDR1 and CYP3A gene polymorphisms on digoxin plasma concentrations of,R96
  7. Association of ABCA1 Gene Polymorphism and Risk Factors with Cerebral Infarction,R743.33
  8. Relatinship of the Small, Dense Low-density Lipoprotein with Coronary Heart Disease and the Stenosis of Coronary Arteries,R541.4
  9. Relationship between the PAPP-A Gene Polymorphism and Ischemic Cerebrovascular Disease,R743
  10. Relationship between the Gene Polymorphisms of Platelet Glycoprotein Ⅲa PLA and Ⅰ a807C/T with Aspirin Resistance,R541.4
  11. Correlation of Aberrant Methylation of p16, APC Gene to MTHFR, TS Genetic Polymorphisms in Hepatic Carcinoma,R735.7
  12. APS patients with type 2 diabetes and the concentration of NO in endothelial progenitor cells PI3K/AKT/eNOS signaling pathway,R285.5
  13. The Expression of DNA Adducts dA in Chronic Atrophis Gastritis, Precancerous Lesions and Gastric Cancer and the Relationship with the CYP2E1 Gene Polymorphism,R735.2
  14. The Relationship between the Plasma Level of HSP70 and Coronary Heart Disease,R541.4
  15. S100A12 in Coronary Atherosclerotic Heart Disease Expression and Significance,R541.4
  16. The Correlation Research between the Gene Polymorphisms of Five CYP450 Isoforms of Enzymes and the Differences of Metabolic Ratio of Cocktail Probes,R969.1
  17. Relationship of C-reactive Protein Genetic and Periodontal Pathogens Polymorphisms between Chronic Periodontitis and Coronary Heart Disease,R781.4
  18. Association between Genetic Polymorphisms of the FP Receptor (Prostaglandin F2α Receptor) Gene and Response to Latanoprost,R775
  19. Analysis of the CYP2C19 Genetic Polymorphism in Uigur and the Association with Effects of Escitalopram,R749.41
  20. The Polymorphism of Parkin Gene in Uygur and Han Patients with Parkinson’s Disease in Xinjiang,R742.5
  21. The Association between BsmI, ApaI Sites in the Vitamin D Receptor Gene Polymorphism and Patients with Type 2 Diabetic Nephropathy Within a Han Population,R587.2

CLC: > Medicine, health > Pharmacy > Pharmacology
© 2012 www.DissertationTopic.Net  Mobile