Dissertation > Excellent graduate degree dissertation topics show

Mesoporous molecular sieve MCM-41 Synthesis and pores of lipase immobilized

Author: WangYanJun
Tutor: ZhangJianGuo
School: Zhejiang University
Course: Biophysics
Keywords: Immobilization lipase mesoporous molecular sieve MCM-41 support
CLC: Q814.2
Type: Master's thesis
Year: 2006
Downloads: 383
Quote: 2
Read: Download Dissertation

Abstract


Enzyme immobilization is one of the most important research fields in enzyme engineering and is cared more and more. Lipase(EC 3.1.1.3, acylglycerol hydrolase) which is widely used in the hydrolyzation, alcoholysis and the synthesis of ester, lactone, polypeptides, macromolecular polymer and the splitting reaction of enantiomorph polymer in the interface of oil-water. However, most free lipases are insoluble and easy to get together in organic solvents. And free lipase itself is not stable and hard to be separated from the reacting system. It helps to widen the interface between enzyme and reactant ,diffuse the reactant, enhance the thermal stability of enzyme, regulate and control the activity and stability to immobilize the enzyme. And the immobilized enzyme also benefits the reaction in the organic solvents and its separation from the products. Besides, it can be reused several times. These strongpoints of immobilized enzyme also play important roles for improving its catalyzing efficiency and prolonging the time of being used.Mesoporous Molecular Sieve MCM-41is a kind of inorganic porous material which has many priorities such as outstanding stability, high mechanical intensity, being nontoxic to microorganism, to resist the acid and the alkali, low cost, long life-span and so on. Because of these good characteristics, MCM-41 is taking on promising future in enzyme immobilization.According to the property and the use of enzyme, the methods for immobilizing enzyme mainly include adsorption, crosslinking, covalent binding and entrapping. The synthesis of mesoporous molecular sieve MCM-41 and immobilizing the lipase on it by simple physical adsorption was studied in the paper. The influence on the enzyme activity and recovery under different immobilizing conditions was also studied. Besides, the thermal stability and the operational stability of immobilized enzyme were discussed.

Related Dissertations

  1. Research on Automatic Detection Algorithm for Substructure Distress of Highway Pavement Based on SVM,U418.6
  2. Research on Autamatic Music Structrue Analysis,TN912.3
  3. Research on the Classification Based on the Reconstruction of Solder Joint,TP391.41
  4. Research on Transductive Support Vector Machine and Its Application in Image Retrieval,TP391.41
  5. Fault Diagnosis Method Based on Support Vector Machine,TP18
  6. Process Support Vector Machine and Its Application to Satellite Thermal Equilibrium Temperature Prediction,TP183
  7. Penicillium Expansum TS414 Lipase in Pichia Pastori: Expression, Purification and Enantioselective Esterification of (R, S)-naproxen,Q814
  8. Studies on Fermentation Optimization, Purification and Enzyme Characteristics of Lipase from Aspergillus Oryzae FS-1,TQ925.6
  9. Directed Evolution of Lipase from Penicillium Expansum FS1884 by Error Prone PCR,Q78
  10. Study on Mental Health and It’s Impact Factors of International Students in China,B849
  11. Designs and Applications of Fuzzy Synthetic Evaluation Models Based on Parallel Algorithms,TP18
  12. Optimization of Fermentation Conditions, Purification, Cloning and Expression of a Cold-active Lipase from Pseudomonas Sp.RT-1,TQ925
  13. The Research on Immobilization of β-galactosidase and Production Technology of Low Lactose Milk,TS252.4
  14. Motor assist system for motorized units,TP311.52
  15. The Relative Research on the Prosocial Behavior and the Perceived Social Support of the Left-in Junior Student,B844.2
  16. Research on Predicting Intrinsic Disorder Protein Structure Based on Supervision Manifold Learning Algorithm,Q51
  17. Cloning and Expression of Organic Solvent Tolerant Lipase Gene from Staphylococcus Saprophyticus M36,Q78
  18. Lipase-catalyzed Transesterification of Lard to Produce L-ascorbyl Fatty Acid Esters,TS221
  19. The Effects of Health Belief Model System Intervention on Prevention of Breast Cancer Realted Lymphedema,R473.73
  20. Citizen Participation of Urban Inhabitant: Structure and Measurement,B849
  21. The Research on the Urban Community Support for the Aged in China,D669.6

CLC: > Biological Sciences > Bioengineering ( Biotechnology ) > Enzyme Engineering > Immobilized enzyme and immobilized cell technology
© 2012 www.DissertationTopic.Net  Mobile