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Research on the Fusion between BMSCs and Neurons

Author: ZhongQi
Tutor: ZhaoShiFu
School: Third Military Medical University
Course: Neurology
Keywords: BMSCs neuron cell fusion rat PEG
CLC: R741
Type: Master's thesis
Year: 2006
Downloads: 85
Quote: 0
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Abstract


It is very difficult to cure the impairment of central nerve system(CNS),because the neuron regeneration ability of brain is poor.Although there are some neural stem cells(NSC) in some places of brain which can diffrentiated into neurons,the repairment capability of the neural stem cells are restricted by the their distribution and amount in the brain.Some researchs of recent years found that some stem cells derived from other organs can differentiate into neurons.In animal experiment,transplanting stem cells such as embryonic neurocytes, cord blood stem cells into the injured brain can partly improve the function of the nerve system. But because of the moral factor and immunologic rejection it is very difficult to apply the other origination stem cells transplantation therapy into clinic.BMSCs can be aquired from oneself and differentiated into neurons in vitro, which have good perspective in the cell transplant therapy of NCS injury. But at present it is hard to make BMSCs express neuron’s characters effectively and stablely.So, it is requisite to find a new effective way to induce BMSCs turn into neurons, then transplant the differentiated BMSCs into injured brain to treat the CNS lesion.Many researchs hint that fuse BMSCs with other type of cells can make BMSCs express the feature of other type of cells. There are no correlated reports about the fusion between BMSCs and cortex neurons.So we conceive to fuse BMSCs with fetal rat cortex neurons to explor the possibility that BMSCs stablely differentiate into regenerable cells which have the characteristics of neurons.The main design of this study is culture and proliferate BMSCs in vitro and separating embryo neurons,then fuse BMSCs with the neurons to observe the proliferation and differentiaton of the fused cells.Methods:Our study divided into three parts:1. culture rat BMSCs and identify the BMSCs by morphology characters,cell surface antigene CD44、CD90、CD45. Then label BMSCs with 5-bromo-2-deoxyuridine(BrdU) before fusion.

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