Dissertation > Excellent graduate degree dissertation topics show

A Study on the Mechanism of Apoptotic Death of Mouse Alveolar Type Ⅱ Cells Induced by Anaphylatoxin C5a

Author: LiBaoSheng
Tutor: CuiSheHuai
School: Third Military Medical University
Course: Internal Medicine
Keywords: acute lung injury anaphylatoxin apoptosis calpain
CLC: R563.8
Type: Master's thesis
Year: 2006
Downloads: 50
Quote: 0
Read: Download Dissertation

Abstract


The ’epithelial hypothesis’ linked apoptotic death of alveolar epithelial cells with the pathogenesis of acute lung injury. It suggested that the epithelial injury caused by apoptotic death of alveolar epithelial cells can trigger sever Inflammatory reaction and in some type of acute lung injury. The important pro-inflammatory factor C5a is prodused when complement system activating. Outcomes of excessive generation of C5a, as occurs in severe infection, when relatively high levels (10–100 nM) of C5a was present in the plasma. Macrophages and endothelial cells produced excessive levels of pro-inflammatory mediators and lead to systemic inflammatory response syndrome and multiple organ dysfunction syndrome. So excessive levels of C5a was an important trigger of ALI. A few of researchers work linked C5a with apoptotic death of some kinds of cells. They found that thymocytes undergone C5a-mediated caspase activation and apoptosis. Anothers reported that C5a delayed apoptosis of human neutrophils. We were intresting at the relationship among C5a ,apoptotic death of cells and ALI . Calpains can activate some apoptotic genes such as Bax gene and lead to apoptosis by promoting the releasing of cytochrome-C from the mitochondrion. On the contrary , a report suggested thatμ-calpain delayed apoptotic death of neutrophil by regulating Bax and subsequent caspase-3 activation. So we supposed that C5a and calpains may have a cooperation on apoptosis. In this study we researched on the apoptotic death of mouse AT-Ⅱcells induced by C5a.Methods:1. Isolation,purification,identification and primary culture of mouse alveolar type II cells: Obtain crude lung cell suspensions by enzymatic digestion. Identificate alveolar typeII cells by electron microscope and alkali phosphatase staining method. 2. Induced apoptotic death of alveolar type II cells by C5a There were there groups : the normal control group, the C5a group and the PD150606 group. After the apoptotic death of AT- II cells was induced, stained cells with FITC/PI and

Related Dissertations

  1. The Study on Cryopreservation and Mechanism of Freezing Injury on the Spermatozoa of Coelomactra Antiquate,S968.3
  2. The Anti-tumor Effect of CADPE and Inducing Apoptosis in Human Gastric Cancer Cells,R735.2
  3. Cloth and β- elemene combined administration of anti-tumor effect and mechanism of celecoxib,R96
  4. TRAIL in the regulation of tumor invasion CD4 ~ CD25 ~ Treg,R730.2
  5. The Study of Lead Exposure on Cyprinus Carpio Ovary Epithelial Cells,X174
  6. Effects of Diclazuril on G3PDH in Second-generation Merozoites of Eimeria Tenella,S858.31
  7. The Effects and Preliminary Study of PCV2 on Ca2+ Signal in Lymphocytes of Piglets,S858.28
  8. Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Sexual Maturity Mice,S852.2
  9. Expression of β-Catenin in Pig’s Ovary and the Effect of β-Catenin on Porcine Granulosa Cells Apotosis and Steroidogenesis Related Enzyme,S828
  10. Induced Combination Apoptosis by FB1 and AFB1 in Vero Cell,S856.9
  11. The Apoptosis Mechanism Induced by Aflatoxin B1 and Deoxynivalenol in Primary Hepatocyte of Cyprinus Carpio,S856.9
  12. Effects of BMPR-IB Gene Silencing by Small Interfering RNA on Apoptosis of Porcine Pollicular Granulosa Cells and Ecpression of BMP Pathy-Way-Ralted Genes,S828
  13. The Effects of Don on Proliferation, Differentiation, and Apoptosis of Chondrocytes in Chicken,S858.31
  14. The Effect of Foxol on Apoptosis of Mouse Granule Cells,S865.13
  15. Study of Melittin on Tumor Inhibition and Part of Mechanism of Human Hepatocellular Carcinoma HepG-2 Xenograft in Nude Mice,R735.7
  16. The Protective Effect of Rat Myocardial Ischemia/reperfusion Injury Following Bone Marrow Mesenchymal Stem Cell Pretransplantation for 1 Week,R542.22
  17. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  18. The Effects of (180F-FDG on the Apoptosis of Eca-109 Esophageal Cancer Cell Line,R735.1
  19. The Signal Mechanism Underlying DON Induced Apoptosis in BHK-21 Cells,S856.9
  20. The Expression and Mechanism of NF-κ B and IL-6 after 90% Portal Vein Ligation in Rats,R657.3
  21. The Study of Mitochondrial Signal Mechanism Induced Apoptosis by Don in Human Colon Cancer Cells,R735.35

CLC: > Medicine, health > Internal Medicine > Respiratory system and chest diseases > Pulmonary disease > Respiratory failure
© 2012 www.DissertationTopic.Net  Mobile