Dissertation > Excellent graduate degree dissertation topics show

Investigation of Compound Huang Dai on Inducing NB4 Cell Apoptosis and Growth Inhibition

Author: YaoXiaoMin
Tutor: WangXiaoBo
School: Dalian Medical University
Course: Pharmacology
Keywords: Nacoscal reaglar Compound huang dai NB4 cell growth inhibitory action apoptosis
CLC: R733.7
Type: Master's thesis
Year: 2006
Downloads: 54
Quote: 0
Read: Download Dissertation

Abstract


Purpose: To study the growth inhibitory action and inducing apoptosis effect of Nacoscale realgar, Qing dai and Compound huang dai on Acute promyelocytic leukemia （APL） cell strain NB4 cell, and to provide the evidence about the mechanism of Compound huang dai treating APL and its main effective ingredients.Methods: This experiment studied Compound huang dai by resolution. APL cell strain NB4 cell was used as vitro model. There were four groups including Nacoscale realgar, Qing dai, Compound huang dai and blank in this experiment. This experiment included two parts:（1） Methylthiazoletrazolium（MTT）assay detected the growth inhibitory degrees afterdrugs treated NB4 cell:At first, the final concentration of Nacoscale realgar ranged from 0.1875 mg·L-1 to 12 mg·L-1, the final concentration of Qing dai did from 0.235 mg·L-1 to 15.04 mg·L-1 and the final concentration of Compound huang dai did from 0.656 mg·L-1 to 42 mg·L-1. These drugs treated NB4 cell respectively, after 24 hours MTT assay was used to detect the growth inhibitory degrees. Then, the correlation of logarithm of drug concentration to the inhibitory rates was analyzed with linear regression and linear equation was obtained, from which the half inhibitory concentration （IC50） came out. Finally compare the growth inhibitory rates of three experimental groups.Then, 1.5 mg·L-1 Nacoscale realgar, 1.88 mg·L-1 Qing dai and 5.25 mg·L-1 Compound huang dai treated NB4 cell respectively and after 6 hours, 12 hours, 24 hours, 48 hours and 72 hours MTT assay was used to detect the growth inhibitory degrees. The significance of differences

Related Dissertations

  1. The Study on Cryopreservation and Mechanism of Freezing Injury on the Spermatozoa of Coelomactra Antiquate,S968.3
  2. The Anti-tumor Effect of CADPE and Inducing Apoptosis in Human Gastric Cancer Cells,R735.2
  3. Cloth and β- elemene combined administration of anti-tumor effect and mechanism of celecoxib,R96
  4. TRAIL in the regulation of tumor invasion CD4 ~ CD25 ~ Treg,R730.2
  5. The Study of Lead Exposure on Cyprinus Carpio Ovary Epithelial Cells,X174
  6. Effects of Diclazuril on G3PDH in Second-generation Merozoites of Eimeria Tenella,S858.31
  7. The Effects and Preliminary Study of PCV2 on Ca2+ Signal in Lymphocytes of Piglets,S858.28
  8. Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Sexual Maturity Mice,S852.2
  9. Expression of β-Catenin in Pig’s Ovary and the Effect of β-Catenin on Porcine Granulosa Cells Apotosis and Steroidogenesis Related Enzyme,S828
  10. Induced Combination Apoptosis by FB1 and AFB1 in Vero Cell,S856.9
  11. The Apoptosis Mechanism Induced by Aflatoxin B1 and Deoxynivalenol in Primary Hepatocyte of Cyprinus Carpio,S856.9
  12. Effects of BMPR-IB Gene Silencing by Small Interfering RNA on Apoptosis of Porcine Pollicular Granulosa Cells and Ecpression of BMP Pathy-Way-Ralted Genes,S828
  13. The Effects of Don on Proliferation, Differentiation, and Apoptosis of Chondrocytes in Chicken,S858.31
  14. The Effect of Foxol on Apoptosis of Mouse Granule Cells,S865.13
  15. Study of Melittin on Tumor Inhibition and Part of Mechanism of Human Hepatocellular Carcinoma HepG-2 Xenograft in Nude Mice,R735.7
  16. The Protective Effect of Rat Myocardial Ischemia/reperfusion Injury Following Bone Marrow Mesenchymal Stem Cell Pretransplantation for 1 Week,R542.22
  17. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  18. The Effects of (180F-FDG on the Apoptosis of Eca-109 Esophageal Cancer Cell Line,R735.1
  19. The Signal Mechanism Underlying DON Induced Apoptosis in BHK-21 Cells,S856.9
  20. The Expression and Mechanism of NF-κ B and IL-6 after 90% Portal Vein Ligation in Rats,R657.3
  21. The Study of Mitochondrial Signal Mechanism Induced Apoptosis by Don in Human Colon Cancer Cells,R735.35

CLC: > Medicine, health > Oncology > Hematopoietic and lymphoid neoplasms > Leukemia
© 2012 www.DissertationTopic.Net  Mobile