|
Objective: Trbutyrin(TB) is the congener of butyric acid(BA). TB can release BA of three molecules in body and maintain the long playing plasma deepness; it can be hopefully used to clinic application. The paper will research whether tributyrin(TB) can inhibit proliferation and induce differentiation or apoptosis in human acute myeloid leukemia cell lines HL-60, K562, human solid tumor cell SGC-7901 and primarily cultured cells from patients with leukemia CNE; will compare the effects treated by TB in leukemia cells and elucidate the mechanism; will supply theoretic and experimental reference for clinic application.Method: HL-60,K562,SGC-7901,NEC cell were used for in vitro culture study. Cell proliferation and viability was analyzed by trypan blue exclusion assay and MTT assay, NBT reduction, benzidine staining, method was used to detect the biological activities of tributyrin at different concentrations and at different time. the P16 activity was studied by immune chemistry methods; The cell cycledistribution was analyzed by flow cytometry , apoptosis was analyzed by DNA ladder.Results: 1、inhibiting of the proliferation :TB inhibited HL-60,K562,SGC-7901 cells proliferation and viability in a tine-dose dependent manner. Treated with TB1.0mM for 72hs the viability of HL-60 ,K562,SGC-7901 cells experimental group was lower than control group cells apparently(p<0.01).2.cell cycle: Treated with TB for 72hs, HL-60, demonstrated accumulation of cells in G1 and / or G2 phase and a decrease of cells in S phase. The response was different between different dose and cell lines.3.inducing differentiation: Treated with 0.5Mm TB for 72hs, induced in differentiation and NBT reduction in HL-60.TB was unable to
|