Dissertation > Excellent graduate degree dissertation topics show

Studies on Tissue Culture and Induction of Tetraploid of Morus Wittiorum Handel-Mazzetti

Author: HeJian
Tutor: LiangGuoLu
School: Southwestern University
Course: Pomology
Keywords: Morus wittiorum Handel-Mazzetti tissue culture tetraploid
CLC: S663.9
Type: Master's thesis
Year: 2006
Downloads: 105
Quote: 0
Read: Download Dissertation

Abstract


The shoot-tips of Morus wittiorum Handel-Mazzetti were used as explants in tissue culture for rapid propagation. And then the method of colchicines inducement combining tissue culture technology has been used to gaining the new polyploidy germplasm of Morus wittiorum Handel-Mazzetti. Additionally, tetraploid germplasm has been obtained which can be used in the breeding of mulberry.The results of experiment as follows:The results in the tissue culture of the Morus wittiorum Handel-Mazzetti showed that: the optimum sterilization method was 10 minutes immersing by scour, 30 minutes washing by water, 40 seconds sterilizing by 75% ethanol, 12 minutes sterilizing by 0.1% mercuric chloride, and washing 4-6 times by sterilized water. The inducing rate of bud initiation is 90%, and the contaminated rate is only 10%, the time of sprouting is 20~27 days. The results indicats that MS medium supplemented with BA 2.0 mg/L + NAA 0.2mg/L + sucrose 30 g/L is suitable for multiplication and 1/2MS+BA 0.1 mg/L+NAA 0.05 mg/L+sucrose 30 g/L is optimal for rooting. And the best rooting rate is 97.4%, the average of each plant has 6.2 roots.On the basis of building the system of in vitro regeneration, the inducing tetraploids of the Morus wittiorum Handel-Mazzetti was carried out by the solution of colchicines. Three methods were adapted: immersing the buds in different concentration solution of colchicines, inoculating buds in the solid medium supplemented with colchicines and in the liquid medium supplemented with colchicines. The results showed that: the method of immersing buds was better than the others, the highest inducing rate was 26.7%. The autopolyploid could be most effectively induced by immersing buds in 0.2% colchicines for 3 days. The optimum concentration of inducing for all the methods was 0.2%.Plant morphology observation of Morus wittiorum Handel-Mazzetti with similar growth stage and environment background suggested that tetraploids are different from corresponding diploids in the forms of leaves, In general, the length and width and thickness of the leaves of tetraploids are bigger than those of diploids, but with smaller leaf index. The characteristic of tetraploids stoma with that of diploid was compared. Stomata of polyploids are larger and spaced father apart on the undersurface of the diploids with less stoma density which is 60.9% than that of diploids. The number of their chloroplasts in the guard cell increased evidently and it is 208.8% than that of

Related Dissertations

  1. Effects of Light Control on Tissue Culture and Photosynthesis Characterristics of Anthurium Andraeanum Lind,S682.14
  2. Study on Rapid Propagation of Energy Plant Euphorbia Tirucalli,Q949.93
  3. Study on the Key Techniche of Tissue Culture of New Phalaenopis ’EVER Greatduble Dragon,S682.31
  4. Studies on the Mechanism and Recovery Measures of Hyperhydricity of Pyrus Calleryana Decne in Vitro,S661.2
  5. Development of Energy-efficient Cultivation System and the Practical Application in Ornamental Plant Tissue Culture,S688
  6. Study on Preservation Method and Strains Variation of Soybean Mosaic Virus in Soybean Production Areas in Anhui Province,S435.651
  7. Study on the Tissue Culture and Seed Germination Rate Modelling of Highbush Blueberry,S663.9
  8. Effect of Light Environment Control on the Growth and Development in Plant,Q945
  9. Studies on Breeding of Tetraploid in Pakchoi (Brassica Rapa L. Ssp. Chinensis L.) Based on Microspore Culture,S634.3
  10. Technology of Propagation in Prunus Maritima,S685.99
  11. Study on Physiological and Biochemical Characteristics of the Transgenic Malus Robusta with LeIRT2,S661.1
  12. In Vitro Tetraploid Induction of Cymbidium Interspecific Hybrids by Colchicine and It’s Identification of Tetraploid Plants,S682.31
  13. Establishment of Rapid Propagation System and Preliminary Mutation on Introduced Bio-energy Willow,S792.12
  14. Research on the Regeneration and the Characters in Physiology and Biochemistry in Citrus Reticulate Blanco Var.kinokuni (Takana) H.H.Hu,S666
  15. The Research of Tissue Culture in Fast Breeding Technology for Osmanthus Fragrans Lour,S685.13
  16. Study on the Introduction, Cultivation and Extension of New Strawberry Cultivars,S668.4
  17. Studies on the Establishment of in Vitro Rapid Propagation Systems of Two Species of Ornamental Ferns,S682.35
  18. Apoptosis of Tetraploid Variants in Citrus Embryogenic Callus,S666
  19. Study on Tissue Culture of Populus Deltoides CL. ’Danhong’ and Eucalyptus Grandis × E.urophylla,S567.239
  20. A Study on Callus Culture and Secondary Metabolites of Schisandra Sphenanthera Rehd. Et Wils,S567.19
  21. In Vitro Culture of Spores and Establishment of Rapid Propagation System of Adiantum Flabellulatum L.,S682.35

CLC: > Agricultural Sciences > Gardening > Fruit trees gardening > Berries > Other
© 2012 www.DissertationTopic.Net  Mobile