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Guben inhibitory Ⅱ on A_ (549) lung cancer cells , mice with Lewis lung experimental study

Author: ZhangGanLin
Tutor: LiXingGuang；LiPing
School: Beijing University of Traditional Chinese Medicine
Course: Clinical in medicine
Keywords: Herbal medicine anti-tumour Lewis lung carcinoma synergetic action immune function
CLC: R273
Type: Master's thesis
Year: 2006
Downloads: 198
Quote: 1
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Abstract

Lung cancer is the most common malignant tumor, morbidity and the mortality from which takes the first place in major cities in China, and the fourth in countrysides, obviously, it is threatening human’s health. The treatment study on lung cancer becomes the focus among the studies of tumor. Strong side effects of actinotheraphy and chemotherapeutics have their application limited. Instead, Traditional Chinese medicine has showing superiority in treatment on lung cancers. In Chinese medicine, lung cancer is a result of stagnation of blood and phlegm due to deficiency of Zang-fu organs. In fact, lung cancer sufferers, especially in late stage, did generally have symptomes with blood stagnation/or blood stagnation in Qi deficiency condition, so the treatment should be to strengthen qi---protecting body, and activate blood---eliminating pathogenic factors, by these two ways, a new balance will be built up again.Gubenyiliu formula II(GII), produced by Beijing City Hospital of Traditional Chinese Medicine, is an effective prescription in treating cancer with syndrome of blood stasis and Qi deficiency, which is generally applied in combination with chemotherapeutics. Clinic datas proved that GII can improve the quality of life and lessen the side effects of chemotherapeutics. In order to study the effective functional ingredients and their acting mechanisms, our experiments individually detected the effects of whole prescription and its functional compositions to the growth of A549 adenocarcinoma cell, aiming at seeking out the specific active ingredient on inhibiting tumor or increasing the effects of chemotherapeutics, and hopefully could guide rational administration in clinic. We also observed the influence of GII(or with CTX) on transplantation tumor and metastatic tumor of mice’Lewis lung cancer, and the effection on immune system, living time and apopotosis.1. In Vitro studies of GII and its functional compositions on A549 lung carcinoma cell and synergistic action in combination with chemotherapyAccording to the actions of each herbs, GII was divided into four groups: Reforcing Qi group(QE)、Promoting qi and activating blood group(QB)、Cultivating kidney and liver group(KL)and Eliminating stagnation and removing lump group(SL). Their aqueous extracts were prepared respectively. MTT assay was used for A549 growth and combinative effect with DDP. Results: Both GII and QB group showed depressive effect on A549 growth. The IC50 was 33.21mg crude drug/ml and 9.55mg crude drug/ml respectively. Both of them had synergistic action with DDP, the multiple was 1.23 and 1.77, complicating index was 1.05 and 0.83. Both of them could obviously inhibit A549 cell growth in G0/G1 phase,the percentages were 84.16% and 86.21%respectively, and reduce the numbers of S phase.

Guben inhibitory Ⅱ on A_ (549) lung cancer cells , mice with Lewis lung experimental study

Abstract

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