Dissertation > Excellent graduate degree dissertation topics show

Molecular Cloning and Expression of an Amidase Gene from Nocardia sp. in Escherichia Coli

Author: XuMi
Tutor: ShenZhongYao；TanTianWei
School: Beijing University of Chemical Technology
Course: Biochemical Engineering
Keywords: Nocardia sp. amidase gene molecular cloning recombinant Escherichia coli
CLC: Q78
Type: Master's thesis
Year: 2006
Downloads: 113
Quote: 0
Read: Download Dissertation

Abstract


Acrylamide and acrylic acid are two kinds of important chemicals widely used in many industrial fields. Amidase is one of the key enzymes received considerable recognition as biocatalysts for microbial-production of acrylic acid and byproduct inhibition of acrylamide. Consequently, the cloning and expression of the gene encoding the amidase was focused and emphasized in this thesis.At first, three different strategies were designed to obtain the amidase gene from Nocardia YS-2002 based on sequence analyses. A novel amidase gene, gene bank accession number of DQ342287, was successfully cloned by PCR. Multiple sequence alignment, phylogenetic tree plotting, catalysis stability prediction, rare codon and hydrophobicity profile analyses, in-cell position determination and secondary structure prediction of the new amidase were further carried out thereafter.Secondly, the amidase gene was inserted into both the plasmid pET-28a and pMAL-p2x. Some recombinant strains were successfully constructed by transforming the plasmids into Escherichia coli BL21 (DE3) and TB1, respectively. Cell culture and amidase induction were further performed under different conditions, and the highest amidase

Related Dissertations

  1. Establishment of Feline Calicivirus (FCV) Reverse Genetics System Using RNA Polymerase Ⅱ and Expression of RHDV VP60 Gene,S852.65
  2. Cloning and Expression Analysis of Cold-Related Genes of Ammopiptanthus Nanus,S793.9
  3. Study on Fermentation Conditions for CPC Acylase Production by Recombinant Escherichia Coli,TQ464.8
  4. Investigation of the Genetic Polymorphism of the Six MiniSTR Loci in Hunan Han Populations,Q3
  5. Molecular Cloning, Expression and Characterization of Wulong Goose MHC Class Ⅰ,S852.4
  6. Cloning、Prokaryotic Expression and Preparation of Polyclonal Antibody of Chicken IL-2 Gene,Q78
  7. Production of Biopharmaceutical Plasmid DNA by Recombinant Escherichia Coli,TQ464.6
  8. Gene Cloning and Polycolonal Antibody Preparation and Tissue Expression Analysis of Prolactin Receptor in the Goose,Q78
  9. Construction and Screening of Human Hepatocarcinoma Cell cDNA Library and Analysis of the Positive Clones,Q78
  10. Cloning and Primary Function Identification of Ribosomal Protein L39 Gene from Culex Pipiens Pallens,R346
  11. Preparation of Stably Nanog-Expressing Fibroblast Cells, Analysis of Their Pluripotency and Construction of Transgenic Cloned Embryos,S823
  12. Large-scale Sequencing of Human Dendritic Cell cDNA Library and Characterization of a Novel Cytokine CX1,R392
  13. Large-Scale Production and Characterization of Recombinant Plasmids,Q78
  14. In Vitro Amplification, Molecular Cloning and Preparation of Nucleic Acid Probe of the Specific Gene Fragment of Porphyromonas Gingivalis and Their Application,R78
  15. Extraction, Purification and Gene Cloning of Alkaline Phosphatase and Effects of Calcium Hydroxide on Its Activity and Gene Expression in Pulp Cells,R78
  16. Amplification, Cloning and Expression of HIV-1 GAG Gene Fragment and the Purification and Identification of the Expressed Product,R346
  17. Amplification, Cloning and Expression of Variable Gene from Anti-human Hepatocarcinoma Monoclonal Antibody and Identification of the Expressed Recombinant Phage Antibody.,R392
  18. Analysis of the Inheritance, Quality-breeding and Encoding-genes of High Molecular Weight Glutenin Subunits Derived from Somatic Hybrid Progenies of Wheat/Thinopyrum Ponticum,S512.1
  19. Effect of Human Neutrophil Defensins and Nucleotides on Regulation of Innate Immunity in Tracheal Epithelial Cells,R392
  20. Molecular Cloning of High Molecular Weight Glutenin Subunits Genes from Aegilops Searsii and Aegilops Bicornis,S512.1

CLC: > Biological Sciences > Molecular Biology > Genetic engineering (genetic engineering)
© 2012 www.DissertationTopic.Net  Mobile