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Electrochemical Studies on the Interaction of Organic Dyes with DNA and Analytical Application

Author: YouJiaYu
Tutor: SunWei
School: Qingdao University of Science and Technology
Course: Analytical Chemistry
Keywords: electrochemistry DNA pyronine B phenosafranine brilliant cresyl blue crystal violet neutral red interaction
CLC: Q523
Type: Master's thesis
Year: 2006
Downloads: 130
Quote: 1
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Abstract


DNA is one of the most important biomacromolecules in life processes as the carrier of genetic information. The studies of some low molecular weight compounds such as anticancer drugs, metal complexes and organic dyes with DNA are crucial fields in mutation detection and clinical diagnostics. In this paper, the electrochemical behaviors of some electroactive dyes such as pyronine B, phenosafranine, brilliant cresyl blue, crystal violet, neutral red etc. and their interaction with DNA on the mercury electrode were carefully studied.This paper is made up of 6 chapters; chapter 1 summarizes the interaction of low molecular weight compounds with DNA; chapter 2 to 6 focus on the electrochemical studies of some organic dyes such as pyronine B, phenosafranine, brilliant cresyl blue, crystal violet, neutral red etc. and their interaction with DNA, respectively. Different electrochemical methods such as linear sweep voltammetry, cyclic voltammetry and UV-Vis spectrophotometric method are applied to the investigation. Based on the changes of their peak currents, potentials and some electrochemical parameters such as the charge transfer coefficient a, the electrode reaction standard rate constant k_s and the diffusion coefficient Do before and after the addition of DNA, it is found that these dyes can bind to DNA and lead to the decrease of reduction peak current. The stoichiometry of these supramolecular complexes such as the binding number and the binding constant are calculated by electrochemical data. The conditions of the binding reaction and the electrochemical determination are optimized. The influences of coexisting substances such as metal ions and amino acids are tested. Under the optimal conditions, the decrease of reduction peak current is proportional to the concentration of DNA and the working curves for the determination of DNA were

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CLC: > Biological Sciences > Biochemistry > Nucleic acid > Deoxyribonucleic acid (DNA)
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