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Transformation of Transcription Factor DREB Gene into the Fast Growing Black Locust Mediated with Agrobacterium Tumefaciens

Author: QinAiGuang
Tutor: LuoXiaoFang
School: Beijing Forestry University
Course: Biochemistry and Molecular Biology
Keywords: Robinia Pseudoacacia Linn gene transformation DREB Stress toletance Agrobacterium tumefaciences
CLC: S792.27
Type: Master's thesis
Year: 2006
Downloads: 154
Quote: 0
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Abstract


Robinia pseudocacia L is one of multipurpose arbor trees with fast-growing, drought resistance, using in soil and water conservation, fodder and nectar sources. The transgenic plant improved its abiotic stress could meet the shortage for reclaiming up the lands in the arid and the saline and alkaline areas.DREB transcription factors specifically bind to DRE element and control the expression of function gene under drought, high-salt and stress. One DREB transcription factor can control the expression of several target functional genes involved in plant tolerance to drought, high-salt and cold stress. Thus, it may be an effective strategy to achieve ideal, multiple and fundamental effect for improving plant stress-resistance by DREB gene transfer.On the establishments of a regeneration genetic transformation system of fast growing variety of Robinia pseudocacia introduced from Hungary, gene transformation system was optimized by GUS coloation analysis and transcription factor DREB gene was transfered into the plant materials of black locust with Agrobacterium-mediation transformation. The shoots produced on the medium supplemented antibiotic, The PCR and Southern blotting analysis showed that the exotic gene DREB was integrated into the fast growing variety of Robinia pseudocacia introduced from Hungary genome. The results in defail are below:1. The explants taken from the raches of compound leaves of, cultured on MS base medium combimed with 6-BA 1.0 mg/L and NAA 0.5 mg/L. Shoot regeneration

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CLC: > Agricultural Sciences > Forestry > Forest tree species > Broad-leaved trees > Locust
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