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Betulinic acid and its derivatives on KB cell apoptosis induced activity and in vivo anti-cancer activity of Exploration

Author: NiMingYu
Tutor: WuYingLiang；MaLi
School: Shenyang Pharmaceutical University
Course: Pharmacology
Keywords: Betulinic Acid (BetA) BetA Derivatives apoptosis KB cell S180
CLC: R73-36
Type: Master's thesis
Year: 2005
Downloads: 125
Quote: 0
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Abstract


This paper focused on the anticancer activity of Betulinic Acid （BetA） Derivatives in vitro and growth inhibition of S180 in vivo.By the way of our lab, MTT assay showed that BetA could inhibit the proliferation, in a dosage dependent manner, of the cells of HT-29、 A2780、 LS-1747、 SMMC-7721、 Bcap-37、 OVCAR-3、 PC-14、 K562、 MA891 and KB cell lines, in which the IC50 of KB cells was the least.Then, we discovered that more than 5 BetA Derivatives have higher anticancer activity to KB than BetA.In the study of the BetA Derivative’s apoptosis of KB cells activity, we observed the typical apoptotic morphological changes after treated with BetA Derivative. Moreover, TUNEL assay detected the apoptotic DNA cleavage and apoptosis cells were also got by flow cytometer after stained with PI, so that the inducing apoptosis activity of BetA Derivativewas approved by morphological, biochemical methods and flow cytometry. Besides, it was also found that when incubated with BetA Derivative, the Mitochondia membrane voltage of KB cells would decrease dose-dependently, which was the characteristic of the apoptotic cell mitochondria and this further affirmed the apoptotic-inducing effect of BetA Derivative on KB cell.The result of the anticancer activity in vivo showed that BetA had excellent ability of inhibition tumor.With these results, we can come to the conclusion that the BetA Derivatives had the anticancer activity through inducing tumor cell apoptosis and could be used as a new compound for anticancer drugs.

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