Dissertation > Excellent graduate degree dissertation topics show

An Oral DNA Vaccine pcDNA3.1+/flk-1n1-7 Against Colonic Adenocarcinoma of Mouse

Author: DuWei
Tutor: ChenMingQing；WangXiCai
School: Kunming Medical College
Course: Oncology
Keywords: VEGFR2 DNA Vaccine Colonic Adenocarcinoma Angiogenesis Metastasis
CLC: R735.35
Type: Master's thesis
Year: 2006
Downloads: 32
Quote: 0
Read: Download Dissertation

Abstract


Objective To construct an oral DNA vaccine pcDNA3.1+/flk-1n1-7 against tumor angiogenesis and investigate the effects and mechanism of the vaccine on tumor development and metastasis in vivo.Methods 1.The recombinant plasmid pcDNA3.1+/ flk-1n1-7 was transformed into attenuated Salmonella typhimurium SL3261 to develop an oral DNA vaccine against the extracellular domain of flk-1.2.Mice were divided into 3 groups and every group was divided into 3 groups （DNA vaccine group, vector group and saline group） and were orally immunized with SL3261 transformed with therecombinant plasmid, pcDNA3.1 （+）, or saline respectively 3 times at 2-week intervals. Then mice in the first group were challenged by subcutaneous （s.c.） injection of colonic adenocarcinoma cells （CT-26） into the right armpit 2 weeks after the last immunization and survival time of the animals was recorded. Mice in the second group were challenged by subcutaneous （s.c.） injection of CT-26 into the right armpit 2 weeks after the last immunization. Flow cytometry （FCM） was used to detect CD3+ cells and CD8+ T cells before vaccination, after vaccination and after the inoculation of CT-26 cells respectively . Twenty-eight days after tumor cells inoculation, mice were sacrificed and analyzed for tumor

Related Dissertations

  1. Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Immature Mouse,S865.13
  2. Effect of β-elemene Combined with Radiotherapy on the Angiogenesis of Lung Adenocarcinoma Xenograft Tumor in Nude Mice,R734.2
  3. Inhibition of Conventional Chemotherapy Combined with Metronomic Chemotherapy on Breast Cancer Xenografts in Nude Mice,R737.9
  4. The Experimental Study of Antiangiogenic and Induction of Apoptosis by Maximum Tolerated Dose Combined with Low-dose Cyclophosphamide in Breast Cancer,R737.9
  5. Maximum Tolerated Dose Combined with Metronomic Chemoterpy on Proliferation and Angiogenesis in Treatment of Breast Cancer,R737.9
  6. Induction of IGF Binding Protein-6 in Vascular Endothelial Cells is a Conserved Negative Feedback Mechanism in Hypoxia-induced Angiogenesis,Q46
  7. Study on Pharmacokinetics and Spatial and Temporal Expression in Mice of DNA Vaccine against Schistosoma Japonicum,S855.91
  8. Mutliple Myeloma-derived Microvesicles: A Novel Way to Promote Myeloma Angiogenesis,R733.3
  9. The Role and Significance of the Expression of TGFA in Breast Cancer Bone Metastasis Cells,R737.9
  10. Inhibition of CD24 Expression to Induce Anoikis in Ovarian Cancer Cells,R737.31
  11. A Rat Model of Tibia Cancer Pain Produced by MADB-106 Mammary Gland Carcinoma Cells,R738.1
  12. The Expression and Clinical Significance of HIF-1α in Renal Clear Cell Carcinoma,R737.11
  13. Locally Advanced Non-small Cell Lung Cancer Recurrence after Radiotherapy and Chemotherapy Related Factors,R734.2
  14. Astrocytes in the study of the role of the adult mouse brain angiogenesis process,R743
  15. Establishment and Improvement of Liver Metastasis Models in with the Splenectomy and Evaluation of Liver Metastases,R-332
  16. Beidan Yifei on Angiogenesis in Rats with Idiopathic Pulmonary Fibrosis Effects of ET-1 and VEGF,R285
  17. Inhibition of Growth and Metastasis of Gastric Cancer by Transfection of B7-1,R735.2
  18. Association Study of mRNA Expression of Runx1 Gene with Tumorigenesis and Lymphatic Metastasis in Human Non-small Cell Lung Cancer,R734.2
  19. Immunoprotective Effects of DNA Vaccine of p55 Gene Fragment Against Pneumocystis Carinii,R392.1
  20. The Effect and Mechanism of BRMS1 on the Invasion and Metastasis of Human Ovarian Cancer Cells,R737.31
  21. Expression of RhoA and EZRIN in Gastric Carcinoma and Clinical Signification,R735.2

CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Intestinal neoplasms > Colon tumor
© 2012 www.DissertationTopic.Net  Mobile