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The Biocompatibility and Angiogenesis Function of the Combination of Adipose Derived Stem Cells and Astragalus Polysaccharide Collagen

Author: ZhangYuXuan
Tutor: HuangJinLong
School: Nanjing University of Traditional Chinese Medicine
Course: Integrated Chinese and Western Surgery
Keywords: adipose derived stem cell astragalus polysaccharides collagen biocompatibility cellular scaffold angiogenesis
CLC: R318.08
Type: Master's thesis
Year: 2013
Downloads: 27
Quote: 0
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Abstract


ObjectiveStudying the feasibility and biocompatibility of the combination of adipose derived stem cells and of astragalus polysaccharide collagen.Research the affection on angiogenesis with adipose derived stem cells combine with astragalus polysaccharide collagen.Explore the interaction and possible mechanism between astragalus polysaccharide collagen and adipose derived stem cells; evaluate the pros and cons of the complex and the existing problems, as well as the influence on subsequent experimental and clinical studiesMethodsCultured adipose stem cells in vitro, and to observe the biological characteristicsPrepare astragalus polysaccharide collagen and detect collagen characteristicsPlanting the adipose derived stem cells into astragalus polysaccharide collagen, observed by light microscopy, the adhesion rate detection cell proliferation by researching the biocompatibility of adiposed derived stem cells and astragalus polysaccharide of collagenMeasure the VEGF content after ELISA assay co-cultured adipose stem cells with the Astragalus polysaccharide collagen complex secretedChorioallantoic membrane assay adipose stem cells with Astragalus polysaccharide collagen composite effect in promoting angiogenesisResults1Study on fat stem cell:cultured adipose stem cell morphology formed well and also growing postive2Biocompatibility of the adipose derived stem cells astragalus polysaccharide gum:adipose derived stem cells adhesion to astragalus polysaccharide collagen bracket, growth and proliferation, adhesion rate:%; adipose derived stem cells in normal growth and proliferation of astragalus polysaccharide collagen scaffold and astragalus the polysaccharide collagen certain stage role in promoting adipose derived stem cells3The astragalus polysaccharide collagen and adipose derived stem cells co-cultured adipose derived stem cells secreted cytokines VEGF inhibition also no significant role in promoting. No significant difference between the control group4Adiposed derived stem cells with astragalus polysaccharide collagen complexes promote chorioallantoic membrane angiogenesis, and significantly higherConclusionA well biocompatibility between adipose derived stem cells and astragalus polysaccharide collagen the astragalus polysaccharide collagen can become a perfect scaffold for adipose derived stem cells. The composite has a role to promote angiogenesis, and its mechanism may be related to cytokines secreted by adipose derived stem cells and transdifferentiation by adipose derived stem cell. Based on these, further study on ASCs can be expecting.

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CLC: > Medicine, health > Basic Medical > Medical science in general > Biomedical Engineering > General issues > Biomaterial
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