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Foot-and-mouth disease is caused by the foot-and-mouth disease virus (Foot-and-mouth disease virus, FMDV) , a worldwide acute infectious diseases of livestock . Which is characterized by the route of transmission is highly infectious , the mortality rate is higher . The use of transgenic plants to produce vaccines is a research hotspot of plant genetic engineering , the production is simple , low cost, safe and convenient to use , easy to store . The carrot planting of broad, nutrient-rich, can be eaten raw , is an ideal study transgenic plants vaccine carrier plants. In this study, by Agrobacterium -mediated to carry O- and A-type foot - and - mouth disease antigen epitope fusion gene O21 - O14 - A21 - HBcAg plant expression vector was transformed carrot callus ; obtained by screening hygromycin resistant callus and resistant plants were regenerated ; histochemical staining of the GUS gene activity detected on the transformed callus and resistant plants and antagonistic plants of the PCR , the PCR-Southern , RT - PCR and other molecular biology . The obtained results were as follows : (1) Establish a large variety of carrots East regeneration system . The best callus induction MS 2,4-D 0.1 mg / L, the differentiation medium was MS medium without any hormone rooting medium was MS NAA 0.2 mg / L . By screening , renewable 156 -resistant plants . Established and optimized carrot Agrobacterium-mediated genetic transformation system . The experiments show that the dry pre-culture and vacuum can significantly improve the conversion efficiency of Agrobacterium concentration , dipping time , co-culture of the time difference and add As not significantly affect the conversion efficiency . Bacterial concentration OD600 0.5 to 0.8 , dip time of 5 to 10 min of incubation time 3 d favorable conversion . Broth concentration OD600 0.3 carrot late screening antibacterial , its regeneration favorable . Hygromycin and carbenicillin confrontational callus regeneration ability was significantly inhibited . GUS staining and molecular biology test results are as follows:
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