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RAPD Analysis on Genetic Relationship of Different Genotypes in Ramie (Boehmeria Nivea L.Guad)

Author: ChenHeQuan
Tutor: PengDingXiang
School: Huazhong Agricultural University
Course: Crop Cultivation and Farming System
Keywords: Ramie RAPD Cluster analysis Similarity coefficient Geographic distribution Kinship
CLC: S563.1
Type: Master's thesis
Year: 2006
Downloads: 99
Quote: 4
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Abstract


Selection of 44 cultivars SDS method , its total DNA sample extraction , as well as improved CTAB three methods of extraction . Worked out for ramie RAPD analysis of fast , easy material culture methods - underground stems sand culture and DNA extraction method --- improved CTAB method . Choose 80 random primers , random amplified total DNA of the tested varieties . 23 primers obtained stable RAPD profiles , the molecular weight of the amplified fragment between the 0.1kb-2.2kb , the number of bands at 2-8 between coamplified 112 strip . Using NTSYS software clustering generate tree maps will be available for the tested varieties are divided into four categories, all kinds of genotypes of each group a certain similarity in the geographical distribution of similarity coefficient of 44 varieties . Ramie PCR amplification conditions and procedures at the same time a part of the optimization and explore . Tested eight varieties of morphological and yield traits and four quality traits survey data were analyzed using SAS software . Former clustering tree generated map will be divided into four categories, all kinds of genotypes of each group there are certain similarities in the geographical distribution of the tested varieties . The latter clustering tree generated map the varieties are divided into four categories , all kinds of genotype of each group has a certain similarity in geographical distribution . Three clustering results , the three have a class , including most of the tested varieties , and most of the varieties of the same class , 34 of the same species in the analysis of molecular markers and quality traits , morphology and yield traits The analysis of the results of 33 of the same species . In addition, these same species in the geographical distribution there are a lot of similarities . Three clustering results similar not only reveal the genetic relationship between the tested cultivars , and confirmed the reliability and accuracy of the results .

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CLC: > Agricultural Sciences > Crop > Economic crops > Fiber crops > Fiber crops > Ramie
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