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Researches on the Relation of Toxoptera Citricidus and Cirtus Tristeza Disease Epidemic

Author: LiZhenNian
Tutor: WangGuoPing；HongNi
School: Huazhong Agricultural University
Course: Plant Pathology
Keywords: Citrus tristeza virus (CTV) Aphid Popular Was toxic time Inoculation time DAS-ELISA IC-RT-PCR
CLC: S436.66
Type: Master's thesis
Year: 2006
Downloads: 71
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Abstract


Citrus tristeza virus (Citrus tristeza virus, CTV) the Citrus tristeza disease is caused worldwide citrus disease, a serious threat to the citrus industry, Brazil and other South American countries once therefore disease hazards so that the citrus industry brink of crash. In recent years, with the adjustment of product structure of China's citrus industry, CTV in some varieties growing trend. Performance has been generally well received by the tristeza virus infection in Zhejiang, Sichuan, Guangxi, some citrus producing areas, grapefruit and orange stem pitting symptoms. CTV spread mainly by grafting and aphids in the field, including the the dominant populations Orange aphid is the most effective dissemination of CTV mediator. Carry out the aphids Citrus tristeza epidemic relations, and extremely important role in the prevention and control of CTV. In order to understand the relationship of aphids with CTV popular test investigation citrus aphid occurrence and damage of the clear advantages of the citrus aphid populations Citrus tristeza virus transmission characteristics of the disease (CTV); orange aphids spread the virus transmission rate impact Biography the poison rate factors analysis, clear aphid in Citrus tristeza epidemic in; well as the detection of the CTV molecular biology of the infected Orange aphid body. Citrus aphid species survey to determine the the Wuhan regional citrus aphids advantage Orange aphid populations. -2005 Fall from the spring of 2004, the first school citrus orchard and Hongshan District built a village of some citrus orchard investigation, sampling single leaf notation on average each leaves aphids 60-500 head. Orange aphid infestation in the surveyed areas, mainly the main spring both a small amount of cotton aphid, of Spiraea orange aphid and green peach aphid occurred. Under different conditions, the Orange aphid transmitted Citrus tristeza virus efficiency are very different. This test proved that the single head aphid transmission efficiency was 8%, 30 aphid transmission efficiency can reach 75%. Discovered through research on different time is so toxic, 10 aphids were toxic time 24h, the transmission efficiency of more than 50%; were drug 4h, pass the poison was only 12%. Inoculation temperature (maximum temperature for reference) aspects of the 29 ° C aphid pass the CTV efficiency is 60%; 35 ° C, only 12% pass poison. States aphids virus effect is not the same age, with the highest rate of adult mass poisoning, followed by 3,4-instar nymphs, the younger states aphid virus transmission rate lowest its feeding capacity is proportional to. Impact biography the poison rate of many factors, the aphids at different developmental stages of a greater impact on the spread of CTV efficiency; efficiency of the different sources of drugs in plants and inoculation of plant varieties and species of aphid transmission of CTV also have an impact. Environmental conditions, primarily the impact of temperature on the rate of aphid transmission, high temperatures cause aphids feeding capacity decreased; and temperature also affect the the CTV virus particles in plants in vivo replication, the general summer under high temperature conditions, the viral content of the plants significantly reduced. Orange aphid carrying CTV detection. Serological testing, DAS-ELISA detection orange aphids infected, detected 100 aphids infected, the result is not satisfactory; molecular biology detection by IC-RT-PCR, can detect a single head orange aphids with toxic situation, high sensitivity.

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CLC: > Agricultural Sciences > Plant Protection > Pest and Disease Control > Horticultural Crops Pest and Disease Control > Fruit tree pests and diseases > Citrus pests and diseases
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