Dissertation > Excellent graduate degree dissertation topics show

Microbial Transformation of Ferulic Acid to Vanillin

Author: ZhengLiRong
Tutor: SunZhiHao
School: Jiangnan University
Course: Biochemical Engineering
Keywords: Rice bran oil foot Vanillin Aspergillus niger Scarlet secret volvatus is
CLC: TQ651
Type: Master's thesis
Year: 2006
Downloads: 353
Quote: 3
Read: Download Dissertation

Abstract


In this thesis, Aspergillus niger ( Aspergillus niger ) CGMCC0774 and scarlet of dense hole bacteria ( Pycnoporus , cinnabarinus ) CGMCC1115 joint conversion of ferulic acid in rice bran oil foot production of vanillin research . The hydrolysis extract of rice bran oil foot ferulic acid is used as a substrate in the production of vanillin . Irradiated by 60Co Aspergillus niger ( A. niger ) SW - 33 , after screening , rescreening , and then re-screened a resistance to higher substrate concentration mutant strain of Aspergillus niger ( A. niger ) CGMCC0774 three times the average shake The bottle was converted to vanillic acid concentration of 2.481 g / L , the molar rate of 71.62% . The Aspergillus niger CGMCC0774 into vanillic acid ferulic acid to generate the optimal conditions : the transition temperature to 37 ° C , speed 150 r / min , the substrate ferulic acid concentration of 4 g / L , pH 4.0 - 4.5 , shake bottled liquid The amount of 140 mL/250 mL . Aspergillus niger CGMCC0774 into ferulic acid generated vanillic acid 15 L and 25 L fermentor scale-up , the flow of substrate vanilla acid 2.24 g / L , the molar conversion rate was 64.6% . The study is a two-step series vanillin from ferulic acid conversion process , Aspergillus niger generated conversion of ferulic acid to vanillic acid conversion solution was filtered to remove the bacterial cells , adjusted pH 5.0 , supplemented with 5 g / L glucose, sterilization , using cultured the good vermilion secret volvatus free bacterial transformation , production of vanillin 0.63 g / l . Research scarlet secret hole bacteria ( P. cinnabarinus ) separation of CGMCC1115 reaction coupled into vanillic acid concentration of 4 g / L and the amount of resin and 20 g vanillin concentration of 2.20 g / L , the molar yield of 60.69% . Obtained after purification of the subjects developed the vanillin crystalline sample 13C isotopic carbon 13C/12C ratio significantly different from Yuchuang the the lignans material chemically synthesized vanillin , with Rhovanil ? Natural ( Natural equate ) similar . Try preliminary study on scarlet secret volvatus cells immobilized into 72 h produced vanillin 0.499 g / L Immobilized cells repeatedly used four times , vanillin concentration decreased significantly , scarlet secret volvatus and immobilized needs further study .

Related Dissertations

  1. Expression of Glucose Oxidase Gene from Aspergillus Niger Z-25 in Pichia Pastoris,Q78
  2. Studys of the Protein Extract and Utilization of Producing Protein Feed from Tea Residues,S816
  3. Screening of High β-glucosidase Producing Strains and the Effect of β-glucosidase on the Bound Aroma in Wine,TS262.6
  4. Effect of Mutation at the Lid Subdomain of Aspergillus Niger Lipase on Its Activity,TQ925
  5. Cloning and Expression of Aspergillus Niger β-glucosidase Gene,TQ925
  6. Research on Inulinase Strains Breeding and Its Fermentation,TQ925
  7. Production and Application of Epigallocatechin Gallate Hydrolase,TQ465
  8. UV Mutation of Pectinase-producing Aspergillus Niger and Proteomic Analysis of Synthetic Regulatory Mechanisms,TQ925
  9. Effect of Different Treatments on the Contents of Free and Bound Gossypol in Cottonseed Cake,S816
  10. Aspergillus Niger β-MANNANASE Gene Cloning and Expression of Prokaryotic and Eukaryotic,Q78
  11. Research of Bovine Prochymosin Gene Expression in Aspergillus Niger,TS252.1
  12. The Optimization of Production, Purfication and Application of Xylanase from Aspergillus Niger S33,TQ925
  13. Differential Kinetic Spectrophotometric (Or Spectrofluorometric) Determination of Some Substances in Complex Systems by Chemometrics,O657.32
  14. Screening of Strain Producing β-Glucosidase and Studies on the Induction Effects of Corncob,Q933
  15. Studies on the Interaction between Vanillin, Metal Ions and Lysozyme,Q50
  16. Breeding a Strain Produced Oils with Cellulose by Protoplast Fusion,Q933
  17. Cloning and Expression of an α-amylase Gene from the Potato and a γ-amylase Gene from the Aspergillus Niger in Pichia Pastoris GS115,Q786
  18. Antibacterial Properties of Ag/Cu Implanted Stainless Steel,TG142.71
  19. Study on the Process of Breeding and Fermentation of High Naringinase-Producing Strains,S182
  20. Modification of Chitosan and Application in Treatment of Phenolic Wastewater,X703
  21. The phytase yield Strain and fusion screening,Q939.9

CLC: > Industrial Technology > Chemical Industry > Spices and cosmetics industries > Spices chemical
© 2012 www.DissertationTopic.Net  Mobile