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Construction of Two-dimensional Liquid Chromatography System and Its Application in Bio-samples Analysis

Author: WangZhiCong
Tutor: ZhaoZhongYi；LiTong
School: China University of Geosciences
Course: Analytical Chemistry
Keywords: Dimensional liquid chromatography System Interface Peak capacity Biological Samples
CLC: O657.72
Type: Master's thesis
Year: 2005
Downloads: 272
Quote: 1
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Abstract


Dimensional liquid chromatography is a recently developed an efficient separation technology, orthogonal 2D systems in resolution, peak capacity than the one -dimensional case has increased , has become a complex system of strong component separation and analysis powerful tool , especially in the life sciences proteome research has been widely applied . This paper is in the \Exchange studies \Thesis describes the basic principles of two-dimensional liquid chromatography , based on the two-dimensional liquid chromatography in recent proteomics research applications to be systematically summarizes , focusing on different interfaces in the form of two-dimensional liquid chromatography system build, and conduct systematic reviews the separation of complex biological samples were analyzed . Paper include : online two-dimensional liquid chromatography system and its evaluation , the offline -dimensional liquid chromatography system construction and application of the special structure of the two-dimensional liquid chromatography system constructed discussed. The first dimension sample components eluted effectively cut into the second dimension is the two-dimensional design of the key system interface . Striping sample loop transfer samples, analysis of samples in parallel columns alternating enrichment , trapping column sample enrichment and overall two -phase column without interface is commonly used in the form of column switching technique . The second chapter analyzes in parallel columns alternately enriched form interface WAX / RP and GFC / RP two types of online online two-dimensional 2D systems as an example of the general method of system building , and the separation of a mixture of standard proteins systematic evaluation . Two kinds of two-dimensional system's total peak capacity of 189 and 225 , respectively , in the one-dimensional model , whether WAX, GFC or RP can not be completely separated from the test sample , in a two-dimensional system has been better separation . However, this interface is composed of two-dimensional system peak

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CLC: > Mathematical sciences and chemical > Chemistry > Analytical Chemistry > Instrument analysis ( physics and physical chemistry ) > Chromatographic analysis > Liquid chromatography analysis
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