Dissertation > Excellent graduate degree dissertation topics show

Study on the Gene Engineering Subunit Vaccine of H7 Subtype Avian Influenza

Author: LiHe
Tutor: RanDuoLiang;MengQingWen
School: Xinjiang Agricultural University
Course: Preventive Veterinary Medicine
Keywords: Avian influenza Hemagglutinin Subunit vaccine Immunity
CLC: S852.5
Type: Master's thesis
Year: 2006
Downloads: 206
Quote: 1
Read: Download Dissertation

Abstract


Content: The complementary DNA of 1.7kb HA gene was prepared from the viral gene RNA by RT-PCR. The amplified fragment was cloned into the plasmid vector pMD 18-T,then sequenced and subcloned into the downstream of the baculovirus transfer vector pBlueBac4.5/V5-His-TOPO and named as pH7HA. The pH7HA was sequenced and co-transfected the sf9 cell with the lined Bac-N-Blue? DNA by the technique of cationic liposome mediated transfection. The recombinant baculovirus was purified by three cycles of plaque assay with the chromogenic substrate X-gal in the low temperature melting agarose and analyzed by PCR. By means of that we obtained several strains of recombinant baculovirus rpH7HA. We confirmed that the target gene fragment was inserted into the baculovirus genome by PCR. SDS-PAGE result showed that the HA gene was expressed in the baculovirus / insect expressing system. Additionally, the Western-blot assay proved the HA protein of H7N1 subtype avian influenza virus AIV having good biological activit.The HA protein was emulsified into the Freued’ s adjuvant vaccine. Fifteen SPF chikens separate into 3 groups:vaccine one time group、vaccine two time group and Control group. H7HA vaccine used to immunize 3- week-age SPF chichens by intramuscle injection 20μ g per chicken, after two weeks, vaccine two time group immunize the H7HA protion again. The SPF chickens were challenged with H7N1 isolate of HPAI virus by the fourth week after the immunization. The result show, vaccine one time group and vaccine two time group protection rate against H7N1 are 40% and 100% . We also examine conditin of virus release, vaccine two time group virus release rate is 0% . We expectation to solve the problem and makes the contribution for our country’s AIV manufactures.We expresse Avian Influenza Virus H7HA gene protion in recombinant baculovirus on large scale.The result indicated that, if we maintenance the rigt temperature 27.5°C, the rotational speed 150rpm, the increase right amount surface active agent l0u/L and suitable cell 2×105~3×105cell/ml may pass the insect/ baculovirus expresses the system to transfer the bottle to obtain lots of protein,which has the biology active goal. This experiment may take this vaccine industrial production the important basis, at the same time also may for next step continue to ferment the pot research to provide the important foundation.

Related Dissertations

  1. The Research of a Novel High Efficiency PFC Circuit Based on One Cycle Control,TM46
  2. Construction of the Recombinant of HSV-2gD Minotope、 HBsAg、 IL-18 DNA Vaccines and Its Inducing Immune Respons in Mice,R392
  3. Isolation and Identification of Low Pathogenicity Avian Influenza (H9) Virus in Henan and Study on the Biological Charactistics,S852.65
  4. Identification of B.melitensis 16M Protein Immunodominant Antigens,S852.61
  5. Development of Subunit Vaccine of Streptococcus Suis Type 2 and Actinobacillus Pleuropneumoniae,S858.28
  6. Applied Research of Hemicell? Feed Additive in Weaned Piglets,S828.9
  7. Effects of Varying Levels of Potassium Supplementation to the Low Salinity Waters or Diets on Growth and Physiological Characteristics of Shrimp, Litopenaeus Vannamei, Boone,S963
  8. Effects of Several Immunostimulants on the Growth Performance, Non-specific Immunity and Disease Resistance of Cobia, (Rachycentron Canadum),S963
  9. AttM Transgenic Colored Calla Lily and Antibodies Preparation of Ahl-Degrading Enzyme AttM,S682.264
  10. Effect of Extremely Low Protein Diet to Meishan Gilt on Its Farrowing Performance and Characteristics and Immunity of Its Offspring,S828
  11. Expression of IBDV Structural Protein VP2 Gene in Bombyx Mori and Its Immune Effect As Subunit Vaccine,S855.3
  12. Infectious Bursal Disease Virus Particle Vaccine Research and Its Immune Efficacy Evaluation,S858.31
  13. Epidemiologic Studies on Duck Circovirus Infection in East China and Development of Monoclonal Antibody Against Duck Circovirus,S858.32
  14. Effect of Oral Immunization with Inactivated Avian H9N2 Influenza Virus on the Local Digestive Tract and Systemic Immunity in Ducks,R392
  15. DNA Prime/Protein Boost of Japanse Edcephalitis Virus NS1 Protein to Improve Immune Response in Mice,R392
  16. Dynamics Behavior of a Stochastic Delayed SIRS Model with Saturation Incidence,O211.6
  17. The Investigation of Mastitis Subunit Vaccine Aginst Stphylococus Aureus and the Adjuvant-IL-6 for Cows,S858.23
  18. Expression and Immunogenicity of GapC Protein of S.agalactiae, S.dysgalactiae, S.uberis,S858.23
  19. Effects of Anti-fetal Immunity on the Pathogenesis of Hypertensive Disorder Complicating Pregnancy,R714.246
  20. Regulational Effects of IL-10 and Morphine on Intracelular Thioredoxin Expression in Immune Cells,R392
  21. Mapping QTLs on Affecting Immunity Traits on SSC10 in a Swine Population,S828

CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Basic Veterinary Science > Serological
© 2012 www.DissertationTopic.Net  Mobile