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Inheritance and Microsatellite Tagging of Resistance to Soybean Mosaic Virus in Soybean

Author: LiHaiChao
Tutor: ZhiHaiJian
School: Nanjing Agricultural College
Course: Crop Genetics and Breeding
Keywords: soybean(Glycine max(L.) Merr.) soybean mosaic virus(SMV) bulked segrgant analysis(BSA) inheritance gene tagging resistance gene SSR marker
CLC: S565.1
Type: Master's thesis
Year: 2006
Downloads: 215
Quote: 2
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Abstract


Soybean mosaic virus (SMV) is one of the most broadly distributed diseases worldwide which causes severe yield loss and seed quality deficiency in soybean (Glycine max (L.) Merr.). International and domestic scholars have conducted many studies on inheritance and molecular marker of genes resistant to SMV strains in soybean.In present study, the major objectives were to study the inheritance of resistance to the newly identified strain SC-11, SC-13 and SC-14, and to identify the microsatellite markers linking to the resistance gene, in order to provide some useful information for soybean breeding for disease resistance and to make preparations for the fine mapping and map-based cloning of resistant gene. The main results were as follows:1. Seven soybean cultivars with resistance to SMV strain SC-11, Kefeng No.1, Pixianchadou, Dabaima, Qihuang No.22, Youbian30, PI96983 and Kwanggyo was crossed respectively with a susceptible (S) cultivar (Nannong1138-2 or Nannong18-6) to determine the inheritance of their resistance(R) reaction to SC-11. Each R parents were also crossed with each other to test the allelism of the resistance genes. The results showed that a dominant gene controlled the resistance to SC-11 in each of Kefeng No.1, Pixianchadou, Dabaima, Qihuang No.22, Youbian30, PI96983 and Kwanggyo, respectively. The absence of S segregation in F2 populations of the crosses PI96983×Kwanggyo and Qihuang No.22×Youbian30, indicated that the single dominant gene in them are alleles at a common locus or very closely linked. Segregation in the F2 populations from Dabaima×Kefeng No.1, Kefeng No.1× Kwanggyo, PI96983×Pixianchadou, Dabaima×Pixianchadou, Dabaima× Youbian30 was consistent with a ratio of 15R:1S. It was inferred that the resistance genes between Dabaima and Kefeng No.1, Pixianchadou, Youbian30; between Kefeng No.1 and Kwanggyo; between PI96983 and Pixianchadou were not at same locus.2. SMV strain SC-14 isolated from Shanxi Province, China, was a newly identified virulent strain and the only one that can infect Kefeng No. 1, a resistance source with a wide resistance spectrum. In present study, soybean accessions, PI96983, Qihuang No. 1

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CLC: > Agricultural Sciences > Crop > Economic crops > Oil crops > Soybean
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