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Pyrophosphate is an intermediate for a variety of metabolic reactions in the living body , has an important role in life processes : pyrophosphate is mainly used as a water softener and in daily life , food additives, etc. , and therefore is of great significance for the the detection of pyrophosphate . Immunoassay and DNA hybridization detection , SNP ( single nucleotide polymorphism ) analysis has important significance in the life sciences , is widely used in biology, medicine and diagnosis . Following the the radiolabeled enzyme linked immunosorbent assay detection methods , the rise of metal nanoparticles as probes chemiluminescence detection principle of chemiluminescence detection of metal nanoparticles dissolved metal ions to the quantitative determination of immune analysis , DNA hybridization , SNP ( single nucleotide polymorphism) , and high sensitivity . But commonly a metal such as Au, Ag nanoparticles dissolve more difficult , based on this , we prepared the more readily dissolve the metal oxide Nano - tri-iron tetroxide , and after dissolution of Fe3 and Fe2 in the sensitivity of the chemiluminescence is also very high, and also can take advantage of the iron oxide magnetic separation and enrichment of analytes , so as to improve the sensitivity of the detection system provides a shortcut . Established a chemiluminescence detection system pyrophosphate , and successfully applied to the detection of pyrophosphate samples ; using prepared by coprecipitation of iron oxide nano particles surface bare (without modification ) , and the use of chemiluminescence method to detect iron oxide molecules , the detection limit (3σ , n = 11 ) to 3.0 x 10-9 mol / L ; poorly water soluble surface exposed iron oxide nano particles , direct application in biochemical experiments other aspects more difficult. Based on this, we use the solvent thermal technology , 1,6 - hexanediamine ( polyacrylic acid) as the amino- (carboxyl ) functional reagent case , the successful synthesis of a surface modified with amino group (carboxyl ) functional group nano triiron tetroxide particles. The modification of an amino group and a carboxyl group not only improves the nano- iron oxide water-soluble , so that it can with the biomolecule coupling, is expected to be used as a dual function probe (detection and separation, enrichment ) Immunoassays , DNA hybridization , SNP ( nucleotide Polymorphism) analysis, and biochemical fields.
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