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Study on the Role of Histone H2B Ubiquitinationin Melosis

Author: TuHuaYu
Tutor: LiWei
School: University of Science and Technology of China
Course: Genetics
Keywords: Histone H2B Ubiquitination Deubiquitination Knockout Meiosis
CLC: Q343
Type: Master's thesis
Year: 2011
Downloads: 30
Quote: 0
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Abstract


Histone translation modifications including acetylation, phosphorylation, methylation, ubiquitination, and glycosylated such. These post-translational modifications in the structure of chromatin remodeling play an extremely important role in the regulation of gene transcription, DNA damage repair and other life processes. As chromatin core protein, histone H2B ubiquitination exist in close contact with other histone modifications between the \H2B ubiquitination is constituted by a series of events, many factors are involved in and influence the process. Found budding yeast H2B ubiquitination need Rad6, Bre1 Lge1 form of the protein complex involved in the catalytic process, Ubp8, Ubp10 two main cells involved in H2B deubiquitination deubiquitinating enzyme The deubiquitination activity also complement each other. Research to show that Lge1 in the opposite role, it can play in the process raise Bre1, and Ubp8 to chromatin but suppressed Ubp8 raising promote Bre1 raising. Histone modifications especially early during meiosis plays an important role in the occurrence of the gametophyte H2B ubiquitination has been shown to participate, but exactly played what role in H2B ubiquitination and deubiquitination also not clear how to adjust its downstream epigenetic process thereby affecting meiosis. In order to understand the histone H2B ubiquitination mechanism modified during meiosis around H2B Pan biotinylated modified several major protein Bre1 Lge1 of Ubp8, Ubp10 from the following areas to study: 1. H2B ubiquitination related proteins we build the C-terminal fusion of GFP yeast cell lines, and then observed by fluorescence microscopy Bre1, Lge1 localized in the nucleus. 2. Construct the corresponding gene-specific knockout yeast strain, to observe the meiosis process study confirmed Bre1, Lge1 gene knockout yeast meiosis, significantly reducing the budding yeast sporulation rate And Ubp8 ubiquitination enzymes Ubp10 go double mutants of the yeast meiosis impact is not significant. Building histone H2B ubiquitination sites mutant (H2B K123R), study H2BK123 sites ubiquitination role in meiosis. Building specific knockout the H2B ubiquitination enzymes Rnf20 (the yeast Bre1 of congeners) knockout vector, the first generation of chimeric mice the specific knock for further construction in the testes and ovaries, in addition to Rnf20 the mouse lines to prepare, which by the H2B ubiquitination enzymes Rnf20 knockout mice to study its function in the testes and ovaries. Through the experiments, we found that the and H2B of ubiquitination affects the process of meiosis, laid the foundation for further elucidate H2B ubiquitination modified mechanism of action in the process of meiosis.

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CLC: > Biological Sciences > Genetics > Genetics subdiscipline > Cytogenetic
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