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Preliminary Study of Trypsin for Metabolism of Deltamethrin

Author: XiongChunRong
Tutor: ZhuChangLiang
School: Nanjing Medical University
Course: Pathogen Biology
Keywords: Trypsin Deltamethrin Gas chromatography - mass spectrometry joint analysis UV - visible spectrophotometry Metabolize
CLC: TQ450.1
Type: Master's thesis
Year: 2009
Downloads: 25
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Abstract


Chemical control of insect vector-borne diseases control strategy. Due to the extensive use of pesticides, leading to the occurrence and development of the insect vector insecticide resistance. The study of the vector insect resistance mechanism is the hot issues of the vector-borne disease control. The biochemical basis of insecticide resistance mechanisms, detoxification ability is one of the main reasons for insect resistance to insecticides. Our laboratory using suppression subtractive hybridization (Suppressionsubtractivehybridization, SSH) and gene chips (microarrays) technology, eliminate the election of each generation Culex mosquito deltamethrin resistant strains isolated in trypsin gene expression by gene transfection and RNAi methods, confirmed that the the trypsin gene for mosquito deltamethrin resistance genes. To further explore the trypsin participation deltamethrin metabolic process, this study uses UV - visible spectrophotometry (UV method) and gas chromatography - mass spectrometry (GC-MS method) to detect trypsin different concentrations deltamethrin degradation; NIST mass spectrometry database comparison, preliminary identification of its degradation products; further analysis of its degradation products toxic effects with upanddown method. The results show that the deltamethrin UV detector measuring the absorption wavelength of 264nm, its metabolites absorption wavelength of 250nm and 296 nm, respectively. GC-MS assay deltamethrin trypsin degradation products (including non-degradation of deltamethrin substrate) retention time the were 37.968min 15.289min, 18.730min. Among them, 37.968min was not the degradation of deltamethrin remaining peak deltamethrin trypsin degradation products were Cyclopropanecarboxylicacid (C9H12Br2O2, CAS number 72345-91-6) and phenoxybenzaldehyde ( 3-Phenoxybenzaldehyde, C13H10O2, CAS number 39515-51-0). Wistar rats acute oral toxicity test, degradation product group LD50 1366mg/kg, deltamethrin group LD50 of 55mg/kg degradation product toxicity decreased by 24.836-fold (p lt; 0.05). This study shows that the trypsin degradation of non-protein-small molecule compounds deltamethrin, preliminary identification of the degradation products toxicity decline. The results of the research to deepen the understanding of insects for insecticide resistance mechanisms and proteases features, and prompts trypsin expected as biodegradable pesticides pollute the environment and agricultural agent candidate molecules, worthy of further study.

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CLC: > Industrial Technology > Chemical Industry > Pesticide Industry > General issues > Basic theory
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