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The Changes of T Lymphocyte Subsets and Costimulatory Molecules Before and After Shuxuetong Treatment in Type 2 Diabetes Subjects with Diabetic Kidney Disease

Author: ChenXiaoHong
Tutor: DongJiXiang
School: Suzhou University
Course:
Keywords: Type 2 diabetes mellitus (T2DM) Diabetic nephropathy (DKD) T cell subsets Costimulatory molecules
CLC: R692
Type: Master's thesis
Year: 2011
Downloads: 14
Quote: 0
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Abstract


Objective: To determine the type 2 diabetes and diabetic nephropathy in patients with T-cell subsets and costimulatory molecule expression levels to explore their type 2 diabetes and diabetic nephropathy development pathogenic role. Methods: 32 cases measured by flow cytometry with type 2 diabetes without kidney disease patients (DM group), 33 cases of patients with diabetic nephropathy (DKD group) and 20 healthy controls (NC group) in peripheral blood T lymphocytes CD3 , CD4, CD8 expression and CD3 T lymphocytes CD4 CD28 T cell subsets, CD8 CD28 T cell subsets and CD4 CD154 T cell subsets expression. Simultaneous determination of the three groups of patients with lipids, fasting glucose, glycated hemoglobin, urinary albumin excretion rate were compared between groups. With \Results: 1, before treatment NC group, DM group, DKD group comparative clinical data: Three groups of patients were no significant differences between lipids (P gt; 0.05). And NC group, DM group and DKD group FBG (6.93 ± 0.86,6.74 ± 1.21), HbA1c (7.77 ± 1.39%, 7.95 ± 1.36%), UAER (9.16 ± 6.47,87.60 ± 48.94) were significantly higher ( P lt; 0.01), but the DM and DKD group FBG, HbA1c was no significant difference (P gt; 0.05), DKD UAER group was significantly higher than DM group (P lt; 0.01). 2 DM group before and after treatment, DKD group of clinical data were compared: DM group UAER after treatment (9.02 ± 8.12), and no significant change compared to before treatment (P gt; 0.05); DKD group after treatment of UAER (82.76 ± 45.08) compared with the previous decreased, but the difference was not statistically significant (P gt; 0.05). Two groups of patients before and after treatment, fasting blood glucose, glycosylated hemoglobin, lipids were not significantly different between (P gt; 0.05). 3, before treatment NC group, DM group, DKD group in peripheral blood T cell subsets: Compared with NC group CD3 T cells (67.47 ± 7.70%) compared, DM group, DKD group (62.51 ± 5.39%, 62.68 ± 6.51%) decreased significantly (P lt; 0.01); with NC group CD4 T cell count (35.08 ± 5.11%) compared,, DM group, DKD group (41.49 ± 4.25%, 44.18 ± 4.84%) was significantly higher (P lt ; 0.01); with NC group CD8 T cells (28.12 ± 3.31%) compared, DM group, DKD group (22.89 ± 2.64%, 21.66 ± 2.89%) was significantly decreased (P lt; 0.01); with NC group CD4 T / CD8 T ratio (1.26 ± 0.17) compared, DM group, DKD group (1.84 ± 0.30,2.08 ± 0.37) increased significantly (P lt; 0.01). DKD group and DM group, CD4 T cell count, CD4 T/CD8 T ratio higher than the DM group (P lt; 0.01), CD8 T cells decreased (P lt; 0.05), but the difference was not CD3 T cells significantly (P gt; 0.05). 4, before and after treatment in DM group, DKD group T cell subsets in peripheral blood compared with the change: After treatment, the number of CD4 T cells in DM group (39.87 ± 4.15%) decreased, CD8 T cells (24.08 ± 3.05%) increased both statistical significance (P lt; 0.05); DKD group CD4 T cell count (41.15 ± 4.44%) decreased, CD8 T cells (23.24 ± 3.12%) were significantly increased and meaningful (P lt; 0.01); DM group and DKD group CD4 T/CD8 T ratio (1.68 ± 0.29,1.79 ± 0.26) decreased significantly (P lt; 0.01). CD3 T cells before and after treatment showed no significant difference (P gt; 0.05). 5, before treatment NC group, DM group, DKD CD3 T cells in peripheral blood of CD4 CD28 T cells, CD8 CD28 T cells, CD4 CD154 T cells, expression levels: Compared with NC group, CD4 CD28 T cell subsets (26.49 ± 2.76%) compared, DM group (29.92 ± 7.13%) increased significantly (P lt; 0.05), DKD group (32.65 ± 6.77%) increased more significantly (P lt; 0.01), and the DKD group and DM group increased significantly and there was significant difference (P lt; 0.05); with NC group CD8 CD28 T cell subsets (18.79 ± 2.73%) compared, DM group, DKD group (16.86 ± 3.52%, 16.47 ± 3.64% ) were significantly decreased (P lt; 0.05), DKD group compared with the DM group was not significantly different (P gt; 0.05); with NC group CD4 CD154 T cell subsets (0.61 ± 0.24%) compared, DM group, DKD group (2.01 ± 0.56%, 2.24 ± 0.67%) were significantly higher (P lt; 0.01), and the DKD group than in the DM group (P lt; 0.05). 6 DM group before and after treatment, DKD CD3 T cells in peripheral blood of CD4 CD28 T cells, CD8 CD28 T cells, CD4 CD154 T cells in comparison: DM group after treatment CD4 CD28 T, CD4 CD154 T cell subsets levels (27.09 ± 5.20%, 1.79 ± 0.58%) compared with before treatment were significantly decreased (P lt; 0.05); CD8 CD28 T cell subsets (17.16 ± 2.35%) increase compared with before treatment (P lt; 0.05). DKD group of CD4 CD28 T, CD4 CD154 T cell subsets (29.85 ± 6.91%, 1.92 ± 0.67%) compared with the previous decline (P lt; 0.05); CD8 CD28 T cell subsets (16.91 ± 1.76%) increase over the previous significantly (P lt; 0.01). Conclusion: 1,2 diabetes imbalance of T cell subsets, abnormal expression of costimulatory molecules, in patients with diabetic nephropathy compared with non-diabetic nephropathy patients express more serious imbalance, abnormal activation more pronounced, suggesting that dysregulation involved in autoimmune Type 2 diabetes and diabetic nephropathy and development. 2, with \treatment can modulate the immune abnormalities, pathophysiological changes in diabetic nephropathy, which relieve symptoms.

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CLC: > Medicine, health > Surgery > Urology ( urinary and reproductive system diseases) > Kidney disease
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