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Influence of Tartary Buckwheat Vinegar and Salicylic Acid Chromium (Ⅲ) Complex on the Gut Microbiota Communities Structure of the Mice

Author: ZhangFeng
Tutor: LiYanQin;LiuBin
School: Shanxi University
Course: Microbiology
Keywords: Intestinal flora TGGE / DGGE Buckwheat vinegar Salicylic Acid Chromium (Ⅲ) Complexes
CLC: R587.1
Type: Master's thesis
Year: 2011
Downloads: 56
Quote: 0
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Abstract


In the 21st century, the rapid economic development, people's living standards improve, people's diet contains a lot of fat and high-calorie ingredients, combined with people's lack of knowledge of healthy living, making glucose regulation or diabetes, obesity or overweight, high blood pressure such as energy metabolism and hyperlipidemia prevalence of the disease increased year by year, become a hazard to human health hidden killer. As the prevalence of obesity and diabetes continue to rise, and even its pathogenesis research has effective control measures without delay. Many studies have shown that, with a huge number of human commensal microbes, intestinal microbial host sugar, fat and other substances have an important impact on the metabolism. Buckwheat has hypoglycemic, lipid-lowering, antioxidant, free radical scavenging and inhibition of tumor and other functions, the preferred food for diabetics. In the first part of the paper used to study the molecular ecology of buckwheat vinegar intraperitoneal injection of streptozotocin (STZ) induced diabetic mice, the treatment of high blood sugar and buckwheat vinegar intestinal flora in diabetic mice structure. Measured with a blood glucose test strips fasting glucose levels in mice experiments, the results showed that: buckwheat vinegar has a hypoglycemic activity. With TGGE / DGGE technical analysis laboratory mouse intestinal microbial community structure variation, the results show that: with elevated blood sugar, a mouse model of community structure has also changed; feeding buckwheat vinegar and positive drugs, blood glucose levels decline and improve the structure of the intestinal flora related. Before and after treatment with regular changes of electrophoretic patterns bands were recovered, cloned, sequenced and found to be the rapid increase in the number of bacteria closely related with hyperglycemia. This is directly related to the development of diabetes. Cr (Ⅲ) is the body maintain glucose metabolism and lipid metabolism of essential trace elements. Chromium as the active ingredient glucose tolerance factor, can increase the number and activity of the insulin receptor. Chromium deficiency will result in energy metabolism, formation of hyperlipidemia, arteriosclerosis, diabetes and other diseases. In the second part of this paper, using PCR-DGGE technique combined with principal component analysis of two acid chromium (Ⅲ) complexes on the high-fat diet induced obese mice fed the therapeutic effect, as well as food and sex on chromium complexes Intestinal microflora structure. The results showed that: acid chromium (Ⅲ) complexes can significantly improve the high-fat diet fed mice the physiological and biochemical indicators that obese mice intestinal microflora structure has been improved and become normal; fat diet on the intestine tract microflora impact than salicylic acid Chromium (Ⅲ) complexes and sex more pronounced. Cecal contents of samples for study: food on the structure of cecal flora is significantly greater than the influence of drugs and sex, which is a stool sample analysis results are consistent; fat diet mice cecum Bacteroides and Bifidobacterium flora structure, but on Lactobacillus little effect; drugs on cecal Bacteroides, Bifidobacterium and Lactobacillus flora structure is not obvious.

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CLC: > Medicine, health > Internal Medicine > Endocrine diseases and metabolic diseases > Islet disease > Diabetes
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