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Objective: To investigate the heterotopic tracheal transplantation , regeneration of rat tracheal epithelial cells form a dynamic process of differentiation and function well epithelium . Methods: The experiment was divided into experimental and control groups , trypsin digestion to remove tracheal epithelial cells , the experimental group perfusion tracheal epithelial cells , normal saline control group , and then two sets of heterotopic tracheal transplantation to SD rats subcutaneously full ring The staurosporine A intraperitoneal injection process . 1, 2, 3, and 4 weeks after transplantation kill rats , remove the trachea . Morphological evaluation of airway epithelial integrity , degree of differentiation , submucosal inflammatory cell infiltration , fibrosis and luminal occlusion situation . Immunohistochemical CK14 , CK18 and CFTR identified airway epithelial cell differentiation ; Ki-67 Identification of epithelial cell proliferation . Scanning electron microscopy and transmission electron microscopy ultrastructural changes . Results: Morphology: one week after the transplant the tracheal wall most of the visible discontinuity squamous coverage , local epithelial the nonciliated structure ; 2 weeks after transplantation , the full thickness of the wall of the airway seen more consecutive squamous epithelium covering partially visible columnar epithelium cilium structure ; 3 weeks after transplantation , the full thickness of the wall of the airway seen continuous columnar epithelial covering local visible cilia structure ; 4 weeks after transplantation , the full thickness of the wall of the airway visible continuous ciliated columnar epithelium covering . The wall of the airway epithelial cells of the control group coverage , inflammatory cell infiltration in the lumen . CK14 and CK18 expression at four time points 1, 2, 3 and 4 weeks after the trachea ; CFTR expression in the four weeks trachea . One week after the strongest expression of Ki-67 , after the expression gradually decreased . Scanning electron microscope : from the first week after most of the wall of the airway epithelial coverage to four weeks to form the fully differentiated pseudostratified ciliated columnar epithelium ; transmission electron microscopy 1 week visible squamous epithelium , four weeks visible structural integrity of the ciliated epithelial cells connected close . Conclusion: rat tracheal epithelial cell infusion to bare heterotopic tracheal transplantation to rats subcutaneously the renewable ingredients of completely and airway epithelial function ; tracheal epithelial regeneration process including : cell wall attachment , cell proliferation, cell differentiation , mature epithelialization .
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