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Screening of Carbomycin Producing Strains and Study of Fermentation Conditions

Author: LiuFeng
Tutor: QiaoJianJun
School: Tianjin University
Course: Pharmaceutical Engineering
Keywords: Acetyl isovaleryl tylosin Heat - resistant Streptomyces Optimization of fermentation UV mutagenesis Microwave Mutation
CLC: TQ465
Type: Master's thesis
Year: 2010
Downloads: 52
Quote: 0
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Abstract


Acetyl isovaleryl tylosin (Acetylisovaleryltylosin, abbreviated as AIV) is a very important tylosin (Tylosin) derivative, which is passed by the heat-resistant Streptomyces ATCC11416 biotransformation Tylosin, Tylosin the 3'- the acetylation of the hydroxyl site, and 4 \change breeding Carbomycin yield strains, fermentation time efficient Tylosin transformed into AIV ready. initially identified heat-resistant Streptomyces fermentation conditions, by the determination of the growth curve, heat-resistant Streptomyces fermentation production Carbomycin to 5 day of its potency and cell dry weight maximum, the sixth day of the current price and bacterial sharp decline to speculate antibiotic degradation is caused by bacterial autolysis double emissions and a reduction in pH of the experiments using UV mutagenesis and microwave mutagenesis method of combining high-yielding strains of heat-resistant Streptomyces Carbomycin breeding. after UV mutagenesis twice, the heat Streptomyces fermentation titer by the the initial 12.34μg/ml to 97.96μg / ml, an increase of nearly 7 times also increased by 6μg/ml to 50μg/ml; Tylosin tolerated through microwave mutagenesis, the heat-resistant Streptomyces Tylosin tolerated from 50μg/ml to mutagenesis 80μg/ml, but its potency is there a certain decline through the optimization of fermentation conditions to determine the fermentation medium carbon and nitrogen sources were starch and peptone, response surface analysis to determine the optimal concentration of 63.5g / L and 35.5g / L, fermentation potency than the original optimal concentration to improve the yield of the addition of 5%. CaCO3 Carbomycin no significant impact, but add isovaleryloxy body material Leu the fermentation titer up 174.03μg/ml, a 19% increase in titer than not add Leu fermentation. before optimization after optimization of fermentation conditions Carbomycin fermentation titer increased nearly doubled to the fermentation broth adding Tylosin and subjected to extraction, the use of HPLC-MS was measured and converted to the fermentation broth Tylosin AIV, conversion rate, and the different amounts on the conversion rate. when Add concentrations below 150 μg, the conversion rate remained stable up, to rise from 74% to 75% when the added concentration of 200μg/ml conversion rate decreased to 72.2%, presumably because more than bacterial tolerance concentration 80g/ml the excessive concentration of Tylosin inhibits bacteria in vivo acetyl transferase enzyme and isovaleryl acylase activity. Tylosin optimum added is about 1.8 times the concentration of bacterial tolerance.

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CLC: > Industrial Technology > Chemical Industry > Pharmaceutical chemical industry > Antibiotics manufacture
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