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Net Signal Spectral Analysis of Multi-component Drugs and Polyelectrolyte Protection of Quantum Dots and Its Application in Nucleic Acid Detection

Author: ZhangDanDan
Tutor: XieHongPing
School: Suzhou University
Course: Pharmaceutical Analysis
Keywords: The ultraviolet spectrum Net signal Sulfamethoxazole Quantum dots Polyelectrolyte Nucleic acid testing
CLC: R91
Type: Master's thesis
Year: 2011
Downloads: 9
Quote: 1
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The first part of this article net signal by UV spectroscopy sulfamethoxazole tablet two effective components while detection methods. Net spectral signal analysis component orthogonal to the spectral subspace spanned by other pharmaceutical ingredients and excipients spectrum, thus eliminating the other components of the analysis of the components of the spectral interference, the net signal will be analyzed component proportional to the concentration, whereby to establish the method of the analysis of the net signal of the pharmaceutical preparation. In this paper, the method to study the UV spectra under acidic conditions of the two active ingredients sulfamethoxazole tablets sulfamethoxazole (SMZ) and trimethoprim piperidine (TMP). SMZ and TMP linear range of 0.48-7.84μg / m (lr = 0.9981), and 0.12-1.5μg/ml (r = 0.9986), the average recoveries were 99.5% and 101.0%, RSD were 1.87% and 3.60%. The method is simple, fast and accurate, and can be used under acidic conditions sulfamethoxazole film SMZ and TMP content, while accurate determination. The second part of this paper is made on the preparation of water-soluble fluorescent nanomaterials quantum dots and polyelectrolyte quantum dot fluorescence protection role in nucleic acid detection research. The mercapto aqueous phase Synthesis of CdTe quantum dots, i.e. CdC1 2 , the Te, NaBH 4 and thioglycolic acid as the raw material, heated to reflux in an aqueous solution synthesis of a series of different color CdTe quantum dots. Condition optimization, better stability green, yellow and orange CdTe quantum dots. Green quantum dot fluorescence quantum yield the highest, at 45 percent. On this basis, as sulfur source, thioacetamide, synthetic emission peak at 612nm red core-shell type CdTe / CdS quantum dots, greatly reducing the time required by the preparation red quantum dots, while further quantum dot fluorescence quantum yield. The method of using an electrostatic adsorption synthesized nanocomposite of the quantum dots of the two poly electrolyte protected, respectively, for the quantum dots - poly diallyl dimethyl ammonium chloride (QD-PDADMAC) and quantum dots - diallyl dimethyl ammonium chloride - polyacrylic acid (QD-DADMAC-PAA). Such nano-composite showing curled, of unequal length, width, the more uniform the flexible strip-shaped form characterized in that the stripe width is about 3-5nm. Such quantum dots - polyelectrolyte nanocomposite premise does not reduce the fluorescence quantum yield of the quantum dots effectively prevent shedding of the ligands of the quantum dot surface mercapto thereby exhibit more obvious than the quantum dot itself time stability, and In certain acids, bases, oxidizing environment acid, alkaline stability, and stability against oxidation, at the same time, also maintained a good biological compatibility of the quantum dots, thus greatly improving the stability of the quantum dots in the specific application. Based on the electrostatic adsorption to the nucleic acid probe P is fixed in the quantum dots - polyelectrolyte surface of the nano-composite strips, prepared QD-PDADMAC-P nano fluorescent probe, and to such a probe is applied to the quantitative detection of nucleic acids. Nucleic acid probes are immobilized on the nanoparticle surface preparation nano fluorescent probe method compared with the conventional use of chemically covalent binding, this method is more simple and fast. QD-based probe of PDADMAC-P, constructed the emission wavelength of the the 545nm QD-PDADMAC with ethidium bromide (EB) fluorescence resonance energy transfer system, the establishment of the quantitative detection of nucleic acids. The linear range of the method for detection of nucleic acid target chain of 154-770nM, and a correlation coefficient of 0.998, the lowest limit of quantification 154nM.

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