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Novel BODIPY Fluorecent Probe for Biomolecules Detection and Its Application in Bioimaging

Author: GongWei
Tutor: TangBo
School: Shandong Normal University
Course: Analytical Chemistry
Keywords: Near-infrared Fluorescent probe Fluoride ion Glutathione BODIPY
CLC: O657.3
Type: Master's thesis
Year: 2010
Downloads: 147
Quote: 0
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Abstract


Organisms by various molecules composed of tens to hundreds of millions of dollars through complex chemical changes in life-sustaining phenomenon. Including proteins, nucleic acids, peptides, free radicals, various ions become substance essential to maintain the physiological functions of the body, plays an important role in Biology, special physiological function and a high degree of biochemical effects. Intracellular glutathione is the most abundant non-protein thiol, it has to maintain the redox balance of the body plays an important role. As organisms one of the essential trace elements fluorine, can not only affect the activity of enzymes can promote the absorption of iron, and is closely related to a variety of diseases. Therefore, the detection of these biologically active molecules having important biomedical value. As chemistry, physics, biology and other disciplines cross for the active substance detection of these life is also diverse. The fluorescence method has high sensitivity, good selectivity and detection limit is low, and has been widely used in analytical chemistry. Means of a laser scanning confocal imaging techniques, fluorescent probes in the case does not destroy the biological activity, real-time detection of these life-active substance. The near-infrared fluorescent probe has the advantages of high sensitivity, wide dynamic range, due to its special photophysical and photochemical properties. Within this range of biological molecules autofluorescent weak, can avoid background interference and to obtain a higher analytical sensitivity. And measurement conditions suitable for the physiological environment of life and is widely used in the field of life science research. This thesis is based on fluorescence resonance energy transfer (FRET) principle of photo-induced electron transfer (PET), fluorescence spectra of the changing nature of biologically active substances with fluorescent probes specific role to achieve the detection of specific bioactive substances, carry out the following two work: (a) for the detection of fluoride ion new near-infrared selenium design and synthesis of fluorescent probes and application design and synthesis through selenium - boron bond connection fluorescent dye the BODIPY and between trifluoromethyl benzene near-infrared fluorescent probe using The change in fluorescence intensity in the probe with fluoride ions before and after the reaction to achieve the detection of the selectivity of the fluoride ion in the near infrared region. Synthetic product was characterized by means of IR, 1H-NMR, 13C-NMR. Probe the excitation and emission at 660 and 690 nm, the linear equation (F-F0) = 117.29911 72.23089 [F-] (10-6 M), a correlation coefficient of 0.9844, the detection limit is: 5.24 × 10-8 M. By confocal laser scanning microscopy, the successful implementation of the probe of the fluoride ion in the human hepatoma cell fluorescence imaging. (B) for mercaptan detection of new near-infrared fluorescence probe design synthesis and applications based on the principle of fluorescence resonance energy transfer design synthesis through disulfide-linked the two BODIPY fluorescent dyes near-infrared fluorescent probes. The glutathione disulfide bond, to achieve the selective detection of thiols such as glutathione specificity interrupted. Preliminary analysis of the spectral properties of the reaction of the probe selective experiments and probes with glutathione.

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CLC: > Mathematical sciences and chemical > Chemistry > Analytical Chemistry > Instrument analysis ( physics and physical chemistry ) > Photochemical analysis ( spectral analysis method)
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