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Determination of Toxic Nitrogen Compounds by Capillary Electrophoresis

Author: WenZhiHua
Tutor: ZhangHongYi
School: Hebei University
Course: Analytical Chemistry
Keywords: Capillary Electrophoresis Acrylamide Nitrate Nitrite Thiocyanate
CLC: O658.9
Type: Master's thesis
Year: 2009
Downloads: 19
Quote: 0
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Abstract


Capillary electrophoresis (Capillary Electrophoresis, CE) with its sample processing simple, fast, small sample and reagent consumption, etc., are widely used in biology, medicine, chemical industry, environment and other fields. Capillary zone electrophoresis capillary electrophoresis separation mode, this article introduces the application of the separation and detection of toxic nitrogenous compounds which. Separation conditions such as pH, concentration, voltage system. The method used for the actual sample analysis has been satisfied with the results. The paper is divided into three chapters, which reads as follows: Chapter 1: This chapter introduces several toxic nitrogen compounds analysis methods, including chromatographic method, a lot of applications, but also in some GB capillary electrophoresis is a alternative method, which has been used in some toxic nitrogen-containing compound analysis. Chapter 2: determination nonaqueous capillary electrophoresis on acrylamide in traditional Chinese medicine. Acrylamide protonated in acetonitrile under the low pH environment in the electrophoretic migration occurs in uncoated fused capillary, 30mmol / L perchloric acid solution in acetonitrile as the background, 20 kV conditions of separated, 198 nm direct UV detection. Days and days of different concentrations of acrylamide corrected peak area relative standard deviation of 0.4-1.7% and 1.1-2.4%, respectively. This method is simple, fast, low-cost acrylamide analysis is used in traditional Chinese medicine. Chapter 3: In this study, using a capillary quantitative determination of non-smokers and smokers saliva nitrite, nitrate, and thiocyanate salt content, pH value equal to 6.0, 20 mmol / L phosphate background electrolyte (ionic strength adjusted to 0.0816 mol / kg Na2SO4). an equal volume of acetonitrile and saliva mixed, and then removed by centrifugation and filtration methods saliva samples proteins and enzymes. Bromate because it is impossible to exist in the original in saliva, and do not interfere with the selected separation of the three ions and therefore was chosen as the internal standard. Nitrite concentration in the range of 0.01 to 1.00 mmol / L was corrected peak area and concentration linear correlation coefficient greater than 0.999, for the nitrate concentration in the range of 0.10 to 6.00 mmol / L was corrected peak area and concentration-line The correlation coefficient greater than 0.999, thiocyanate salt concentration in the range of 0.05 to 2.00 mmol / L was corrected peak area and the concentration of the linear correlation coefficient greater than 0.999. Electrophoretic separation process is completed in ten minutes. The proposed method because of its a simple sample process and the relatively low operating costs, and provides an alternative method for the determination of these ions in the saliva.

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CLC: > Mathematical sciences and chemical > Chemistry > Analytical Chemistry > Separation of elements and compounds > Other
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