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Objective: To study the pupa extract the fat rat liver fat content and fatty acid. : SD male rats 100, the adaptive feeding a week after 12 hours of fasting, weighing, take the tail blood samples serum triglycerides, low-density cholesterol lipoprotein, high-density cholesterol lipoprotein and total cholesterol. Body weight and serum triglyceride level rats were randomly divided into nine groups: (a) to prevent the low-dose group fed a high fat diet, each administered to pupa extract 1.5 mL / kg · d. (B) prevention dose group fed the high fat diet, each fed to the pupa extract objects 3.0 mL / kg · d. (C) the prevention of high-dose group, were fed a high fat diet, each fed to the pupa extract objects 4.5 mL / kg · d. (D) in the treatment of low-dose group, were fed a high fat diet, the 5th week, each administered to a pupa extract 1.5 mL / kg · d. (E) the treatment dose group fed with high fat diet five weeks, each fed to the pupa extract the matter 3.0 mL / kg · d. (6) in the treatment of high-dose group, were fed a high fat diet, the 5th week, each administered to a pupa extract objects 4.5 mL / kg · d. (7) high-fat control group (79% of the normal diet, 1% cholesterol, 10% yolk powder, lard 10%), the 5th week, each gavage to distilled water and 1.5 mL. (8) positive control group was fed a high fat diet, the beginning of the first five weeks, each fed to evening primrose oil 3.0 mL / kg · d. (9) blank control group was fed a normal diet, taken four weeks after the end of blood verification fat rat model built. Experiment after six weeks, the rats were sacrificed and the livers refrigerated at -80 ° C. In each group were randomly selected five liver, dry weighed Soxhlet extraction method to extract the liver fat, the determination of the fat content. The resulting esterified fat than its principal fatty acid composition determined by gas chromatography under the following conditions. Inlet temperature: 250 ° C, detector temperature: 260 ° C, 30M × 0.25um × 0.32mm (id) FFAP capillary column. Column temperature using the temperature program: starting temperature of 100 ° C, maintained for 2 min, 20 ° C / min of heating rate rose to 200 ° C, increased to 210 ° C and a heating rate of 1 ℃ / min, then 3 ℃ / min heating The rate rose to 240 ° C and maintained for 3 min; carrier gas was nitrogen with a flow rate of 50mL/min split ratio of 1:40, makeup 40 mL / min. RESULTS: In the group of liver fat content comparison, the prevention group, the treatment group, compared to high-fat control group and positive control group with the control group, there were significant; positive control group than the other groups of fat The content should be high, and the treatment of each group were compared with the prevention group was not statistically significant. Palmitic acid, oleic acid and DHA constituent ratio was not statistically significant between the groups. Myristic acid in fat control group, positive control group, prevention group, the treatment group and the control group compared, statistically significant, but does not maintain statistics on the prevention group and treatment groups compared significance. In the prevention group, treatment group, fat control group and blank control group stearic acid constituent ratio, statistically significant; the prevention dose group, the prevention of high-dose group, treatment group and positive control group, statistically significant. Compare to prevent the low-dose group and the control group, the constituent ratio between linoleic acid in the prevention of the low-dose group and the control group, a difference. Statistical analysis, EPA constitutes a prevention group than in the treatment groups, fat control group and blank control group comparison, p <0.05, statistically significant; the prevention dose group, the prevention of high-dose group, the treatment of various comparison of the group and the positive control group, P <0.05, statistically significant. Conclusion: 1. Pupa extract can reduce the fat rat liver fat content; evening primrose oil has increased liver fat content. 2. Pupa extract may improve fat constituent ratio of rat liver fatty acid such as myristic acid, stearic acid, linoleic acid and EPA, especially in the prevention of high-dose group, but no change of palmitic acid, oleic acid and DHA constituent ratio.
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