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The Energy Study of NRK Cells during Cytokinesis

Author: YangFang
Tutor: AnMeiWen
School: Taiyuan University of Technology
Course: Biomedical Engineering
Keywords: Cytokinesis Cell deformation ATP Energy
CLC: S917.4
Type: Master's thesis
Year: 2010
Downloads: 6
Quote: 0
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Abstract


Mammalian cell cytokinesis is a a typical mechanical process . Large deformation membrane , cytoskeleton frequently in large numbers gathered depolymerization , these behaviors are associated with the energy metabolism of the cells in the process of cytokinesis . ATP as a major energy carrier in the cell , cytokinesis large deformation process and the redistribution of the cytoskeleton to provide a source of energy . So understanding is of great significance in the relationship between cytokinesis mechanical deformation energy . The adherent growth of normal rat kidney epithelial cells (NRK) as the research object , using flow cytometry and fluorescein luciferase assay technology , ATP content NRK cells in different cell cycle phases , from the energy point of view explore the cleavage furrow membrane passive deformation process of cytokinesis and cytoskeletal redistribution active deformation mechanics process . The main conclusions of the work are as follows : 1 , TdR double- blocking synchronization method and serum-starved NRK cells , different cell cycle phase cells , cell cycle analysis by flow cytometry , groups of cells in different cell cycle when phase of percentage . Fluorescein luciferase assay determination of ATP content of the cells at different cell cycle phases . The results show that : the control group and TdR double- blocking group ATP content is a significant difference compared with the serum-starved cells TdR double- blocking 1h 4h cellular ATP content . 2 , according to NRK cell membrane deformability cytokinesis morphological parameters , the energy consumption of membrane deformability of the cytokinesis phase theoretical calculations , found that the increasing trend in the energy consumed by the cell membrane in the process of cytokinesis , and membrane deformability consumption the total energy accounted for about 2% of the cytokinesis phase cells to provide energy . NRK cells in the process of cytokinesis furrow constriction force is calculated , and its the active deformation energy consumption analysis . The results found that the number of myosin II motor calculation of the furrow constriction energy consumption is about the cytokinesis process of membrane deformation of 0.1% of the energy consumption , the use of close to the cleavage furrow at the cell membrane radial direction of the surface tension , the constriction of the cleavage furrow the energy consumption is about 10 % of the energy consumption cytokinesis membrane deformability .

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CLC: > Agricultural Sciences > Aquaculture, fisheries > Aquatic basic science > Aquatic Biology > Aquatic Zoology
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