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Screening and Preliminary Analysis of Differentially Expressed Genes in the Latex of Super-high-yielding Rubber Tree Compared with Its Average Counterparts

Author: XiaoXiaoHu
Tutor: TangChaoRong
School: Hainan University
Course: Biochemistry and Molecular Biology
Keywords: Super high-yielding rubber trees Silver stained cDNA-AFLP TDF Differentially expressed genes Yielding mechanism
CLC: S794.1
Type: Master's thesis
Year: 2009
Downloads: 58
Quote: 1
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Abstract


Natural rubber (cis-1 ,4 - polyisoprene) has excellent elasticity, stretch resistance, insulation resistance and thermal stability is an important industrial raw material and indispensable strategic materials, China's serious shortage Most rely on imports. Because it is in non-traditional rubber planting area, China's natural rubber industry is a strictly resource-constrained industries, currently available for expanding rubber planting area has been very limited, a substantial increase yield per unit area has become China's natural rubber industry development The only reliable way. Xishuangbanna Reclamation found in our super high-yielding rubber trees per plant, yield an ordinary rubber trees more than 10 times for the mechanism of high-yielding rubber trees provide a valuable experimental material. Parse these super-high-yield rubber tree plant molecular mechanism for rubber production of large area to reproduce the phenomenon of super high-yielding foundation, but also for the rubber tree Hevea breeding and production of high-yielding technology innovation to provide a theoretical basis and technical guidance. In this paper, PR107 super high-yielding rubber tree plant and its adjacent control strain for the material, the use of latex cDNA-AFLP technique screening differentially expressed genes, separated by differences in the sequencing of further identification and bioinformatics analysis, combined with the rubber biosynthesis genes expression analysis, preliminary explores super high-yielding rubber trees possible mechanism. The main results are as follows: 1. Establish a suitable rubber tree latex transcriptome analysis of silver-stained cDNA-AFLP technology is based on the existing conditions of the laboratory, in the classic cDNA-AFLP technique based on the improvement and optimization, through a combination of appropriate restriction select the optimal parameters for the PCR reaction and silver staining results optimization experiments, a set of suitable rubber tree latex transcriptome analysis of cDNA-AFLP silver staining technique. This technique has the advantage of high sensitivity, good reproducibility, theoretically rubber tree latex covering over 90% of the transcriptome. 2 fully screened in the ultra-high-yield rubber trees and ordinary rubber tree latex significantly differentially expressed genes with our own established silver-stained cDNA-AFLP technique, the system comparative analysis of the ultra-high-yielding rubber trees and common strains of latex transcriptome were screened two a restriction enzyme on all 384 pairs of primers, and ultimately get the 453 confirmed genes differentially expressed transcripts fragment (TDF), of which 247 to get functional annotation, these TDF involved in cellular metabolism, transcription and translation, defense and stress response, protein degradation and cell signal transduction, and only two directly involved in rubber biosynthesis. On preliminary analysis of differentially expressed genes that super high-yielding rubber tree milk ducts active cell metabolism and good stress response mechanism may be the main reason for its high yield. 3 Comparative analysis of rubber biosynthesis gene in different strains yield per plant and the expression level of the rubber elongation factor (REF), small rubber particle protein (SRPP) and cis-isopentenyl transferase (CPT) and other 10 genes directly involved in rubber biosynthesis, but most of them in the expression level of latex rubber tree plant (super high-yielding plant and general control strain) and strain (Reyan 8-79, Reyan 7-33-97 and PR107) production glue level is not positively related to further illustrate the rubber tree may not be the main reason for super high-yielding rubber biosynthesis link.

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CLC: > Agricultural Sciences > Forestry > Forest tree species > Special use of broad-leaved class > Rubber tree
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