Dissertation > Excellent graduate degree dissertation topics show

Analysis and Pharmacokinetics Study of Coumarin Compounds in Radix Angelicae Dahuricae

Author: ZhangJianPing
Tutor: ZuoLiYan
School: Suzhou University
Course: Pharmacology
Keywords: Radix Angelicae dahuricae courmains HPLC Duliang capsule Quantification Pharmacokinetic LC/MS/MS BBB Brain distribution
CLC: R285.5
Type: Master's thesis
Year: 2011
Downloads: 167
Quote: 0
Read: Download Dissertation

Abstract


Objective: 1. To establish an HPLC method for the quantification of xanthotoxol, bergapten, imperatorin and isoimperatorin in Radix Angelicae dahuricae by HPLC; To establish a liquid chromatography electrospray ionisation–tandem mass spectrometry (LC/MS/MS) method used for the investigation of the pharmacokinetics of 3 coumarin components ( imperatorin, cnidilin and isoimperatorin); and investigate the distribution of the coumarin components in mice brain.Methods: An HPLC method was used to simultaneous quantification of coumarin components in Radix Angelicae dahuricae extract. and Duliang capsules; A liquid chromatography–tandem mass spectrometry (LC/MS/MS) method was used for simultaneous determination of imperatorin, isoimperatorin and cnidilin in rat plasma. With the homogenation of brain tissue, the LC/MS/MS method was used to investigate the concentration of brain tissue and blood with respect to time as to reflect the distribution of the coumarin components in mice brain and the blood.Results: An HPLC method has been established for simultaneous quantification of coumarin components in Radix Angelicae dahuricae extract and the precision, reproducibility, The LC/MS/MS method used for the analysis of biology samples and pharmacokinetic studies of coumarin components: The method was then applied in pharmacokinetic studies of imperatorin, isoimperatorin and cnidilin in rats after oral administration of Radix Angelicae dahuricae extract. It showed that imperatorin, cnidilin and isoimperatorinin could permeate the rat blood brain barrier and show a higher distribution in brain tissue after oral administration of Radix Angelicae dahuricae extract.Conclusion: The HPLC method established in our lab proves to be simple and feasible which could be used to quantification of the courmain components in Radix Angelicae dahuricae and its formulations. The LC/MS/MS has been established to simultaneous quantification of imperatorin, cnidilin and isoimperatorin in rat plasma and its application in the pharmacokinetic studies of the 3 active courmain components after oral administration of Radix Angelicae dahuricae extract in rats. This method proved to be sensitive and specific, and precision. Imperatorin, cnidilin and isoimperatorinin could permeate the rat blood brain barrier and show a higher distribution in brain tissue after oral administration of Radix Angelicae dahuricae extract.

Related Dissertations

  1. Cloning and Expression of CHS and CHI Genes and Their Regulation on the Accumulation of Flavonoids in ’Cara Cara’ Navel Orange (Citrus Sinensis Osbeck) and ’Guoqing NO.4’ Satsuma Mandarin (Citrus Unshiu Marcow),S666.4
  2. Study on the Pharmacokinetics of Xiaoaiping and Chlorogenic Acid in Rats and the Quality Standard of Xiaoaiping Preparations,R285
  3. A Study of Household Handles Design from the Perspective of Kansei Engineering,TS664.01
  4. Isolation Entophytes and Study on the Fermentation of Bacillus Subtilis tiD7 from Rehmannia,TQ461
  5. Development of Detection Methods of Effective Biological Components from Tea by High Performance Liquid Chromatography and Their Applications in the Analysis of Fenghuang Oolong Tea,TS272.7
  6. Studies on the Residues of Bromoxynil and Mesotrione in Maize and Soil,S481.8
  7. Cucumber Growth Promotion and Wilt Disease Suppression of Bacillus Subtilis SQR9 and Their Mechanisms,S436.421
  8. Production of Monoclonal Antibodies Against Thiabendazole and Development of Heterologos Elisa,S482.2
  9. The Yunnan ten kinds of pepper antioxidant and antibacterial activity of the research,S641.3
  10. Determination Method and Extraction Purification of Terpenoid Indole Alkaloids in Catharanthus Roseus,R284
  11. Study on Synthetic of BINOL, Amino Acid and Derivatives Molecularly Imprinted Polymers and Their Application in the Chiral Resolution,O631.3
  12. Detecting Melamine in Feed by High-Performance Liquid Chromatography,S816.17
  13. The Method for the Determination of β2-agonists Residues in Foodstuff of Animal Origin by HPLC-MS/MS,TS207.3
  14. Analysis of Effective Biological Components in Chinese Commerical Tea Drinks,TS272.7
  15. Studies on the Detection Methods of Virginiamycin in Feed,S816.17
  16. Extraction Condition of Paeoniflorin from Paeonia Lactiflora,R284.2
  17. Studies on the Detection Methods of the Tetracyclines Residues in Egg,S859.84
  18. The Study of the Influence of BMS-345541 on Motor Function of Hind Limbs and the Infiltrating Leukocytes after Spinal Cord Injury in Rats,R651.2
  19. An Observation of the Concentration Changes of MTX in Maternal Milk and Serum in Partial Placenta Increta,R714.22
  20. Injection with four commonly cefathiamidine Compatible Stability of transfusion,R942
  21. Application IP-RP-HPLC method study of creatine phosphate in mice pharmacokinetics and metabolic disposition,R96

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology
© 2012 www.DissertationTopic.Net  Mobile