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Grifola frondosa is a rare edible Medicine Fungi has attracted the attention of researchers. Grifola frondosa polysaccharide can assist in anti-cancer therapy, boosting the body's immune function. Has a small toxic the edible fungi biotransformation selenium also can improve the effectiveness of the body's immune system. Rice bran is a by-product of rice processing, large output, to be deep-seated transformation, in order to enhance its value. In this study, the use of edible fungus Grifola frondosa in rice bran medium liquid culture production grifolan and enrichment of organic selenium, Grifola frondosa polysaccharide production and depth conversion of organic selenium use rice bran collection requirements to be addressed to form new ideas, new technology, new results, and to take advantage of cheap raw materials for large-scale industrial production of specific health food lay the foundation for this study, some of the theoretical and practical value. In this study included the following major elements: (1) study frondosa in rice bran as a main component medium the possibility of the liquid fermentation polysaccharides, the composition of the use of rice bran and rice bran medium. Strains using laboratory were irradiated by UV selected the Grifola frondosa rice bran medium adaptive bacteria. Formulated basal medium: potato (a cook fluid) 200g / L, potassium dihydrogen phosphate 1.5g / L, magnesium sulfate 0.75 g / L, vitamin B 1 10mg / L. Starting from the basal medium, the different carbon sources, nitrogen sources, the amount of addition of glucose (0.5% to 2.5%), respectively,, bran added amount (1 to 5% (by weight)) and the like single factor test, select the added amount of glucose ( 1 to 2%), the addition amount of rice bran (4 to 6%) and peptone added amount (0.3 to 0.5%) three factors L 9 (3 4 ) n cross test, and as the marker of Mycelial dry weight and polysaccharide content. Frondosa the liquid fermentation process in a basic medium optimal additional additives and amount are as follows: rice bran 40 to 60G / L glucose, 15g / L, peptone 3 to 4G / L,. Mycelium polysaccharide 281.3mg/100mL, the mycelium average yield value 1246.7mg/100mL. The test results showed that can rice bran as a major medium component replacement or less with glucose and other carbon sources, to reduce costs. Basic medium can not add the potatoes. The rice bran various use mycelium polysaccharide impact is not great. (2) studied the external conditions of the liquid fermentation polysaccharide frondosa as a main component in the optimization of the rice bran medium. Different inoculum size (4 ~ 12mL), the amount of sample loading (50 to 150mL), incubation time (8d), incubation temperature (22 to 34 ° C), shaking speed (90 ~ 210r/min) five The factors affecting the single factor test, and as the marker of Mycelial dry weight of mycelium polysaccharide content. The of Grifola frondosa liquid culture suitable culture conditions: incubation time 5d, incubation temperature of 28 ℃, shaking speed 120 ~ 150r/min, inoculum size 8%, sample loading capacity of 20% (250mL cone shaped bottle). (3) research frondosa in rice bran as a main composition of the medium enriched organic selenium liquid fermentation and production of polysaccharides. Add the amount of different concentrations of selenium selenium single factor test. Accordance with U 12 * (. 12 10 ) uniform design method test, examine selenium X 1 , rice bran X 2 , glucose X 3 and rice bran processing way X and the amount of mycelial growth, mycelial polysaccharide content, organic selenium enrichment relationship uniform test results regression analysis using SPSS software. Using multiple linear regression and quadratic polynomial regression to obtain test results on the uniform design the mycelium dry weight Y 1 , polysaccharide content Y 2 , the selenium content of Y < sub> 3 , selenium-enriched rate of Y 4 regression equations were: Y 1 = 0.070 X 2 0.234 X 3 0.209; Y 2 = 0.024 X 2 0.087X 3 0.046; Y 3 = 0.034X 1 X 3 -0.009 X 4 0.118; Y 4 = 0.003X 2 0.559X 1 X 3 -0.006X 2 X 3 0.022. (4) expanded trial 18L fermenter. Optimization of fermentation medium, the amount of sample loading 15L, fermentation temperature 28 ± 1 ° C, ventilation 0.8v/v/min, stirring speed 150r/min the fermentation tank gauge pressure 0.05MPa conditions 5d. Fermentation time is about 2d the mycelium crude polysaccharide content reached the maximum as 631mg/100mL. Grifola frondosa enrichment of selenium in rice bran-based medium, and at the same time the production of the metabolic processes of the polysaccharide amplification test, laid a theoretical foundation for the formulation parameters and control indicators enlarge production. (5) of the mycelium polysaccharide extraction conditions optimized design considerations, on the basis of single-factor test, orthogonal experiment L interaction 16 (2 15 ), range and variance analysis, extract of mycelium polysaccharide best experimental conditions: extraction time 90min, extraction temperature of 75 ° C, extraction times 2 times, the ethanol concentration of 67v / v . (6) Purification and properties of a DEAE-52 cellulose chromatography analysis of the crude polysaccharide. Three pure polysaccharide component, of pure polysaccharide component analysis of physical and chemical properties, and the analysis results obtained sample is indeed polysaccharide samples, and are free from starch, amino acids, peptides and proteins. Meanwhile, in the 4000 ~ 400cm -1 interval infrared spectroscopy scan analysis, and infrared spectroscopic analysis, it can be seen that after separation and purification, the resultant polysaccharide having a generally more saccharide characteristic absorption peaks.
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