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Study on Cryobiological of Porphya Haitanensis in Cold Preservation

Author: WengLin
Tutor: ChenChangSheng
School: Jimei University
Course: Aquaculture
Keywords: Altar seaweed Thallus Strain Hypothermia Grow Survival Refrigeration
CLC: S984.4
Type: Master's thesis
Year: 2007
Downloads: 100
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Abstract

Thallus strain the P. haitanensis (Porphya haitanensis) and strains seedlings as test material. Explore the different size of the frond frond moisture content, temperature factors of the biological effects of low-temperature refrigeration P. haitanensis; study the biological effects of the the altar seaweed protective agent liquid refrigerated; research altar seaweed refrigerated network applications and refrigerated altar seaweed yield and quality analysis. The results showed that: 1. From haitanensis survival and growth point of view, the altar seaweed refrigerated appropriate moisture content between 10% to 30% to between 10% to 15% of the best. The size of the altar seaweed 1cm and 3cm experimental group, and restore speed train, the growth rate is also better than other algae body size experimental gradient group, refrigerate after sexual maturity in the same incubation time is not easy. Room temperature stored airtight thalli moisture saved survival little effect; saved in 17,20,23,26,29 ° C lower seal, the lower the temperature, the better preservation effects, wherein 17 ° C under save 10d , Cell Death minimal. Low temperature refrigerated experiments, as the temperature decreases, the survival rate after the altar seaweed refrigerated increases; growth rate of -20 ° C and -25 ℃ experimental group refrigerated after day length, day weight growth rate compared to other temperature experimental group higher than the 20% to 40% range. Refrigerated frond recovery related to the survival rate and temperature of the culture, the survival rate and the highest growth rate at 20 ℃ frond survival rate of 100%. Refrigeration time, when the altar of seaweed leaf water content ranged from 10 to 15%, the survival rate of the refrigerated 10d and 30d are up to 100%; refrigerated 720d altar seaweed survival rate was 78.0%. With refrigerated forum seaweed time extension, the survival rate after its refrigerated gradually reduced. At room temperature, under different concentrations of intracellular protective agents (glycerine, DMSO) than extracellular protective agent (glucose, sucrose) greater seedling survival; from the role of a single refrigerated protective agent point of view, glycerol the the refrigerated protection is better than DMSO, seedlings from 20% to 25% glycerol protective agent under refrigeration 30d, the survival rate was 100%; glucose and sucrose as single refrigerated protection agents, refrigerated 15d seedlings all died; through orthogonal experiment showed that protection agents (glycerol DMSO) mixed refrigerated haitanensis, 20% glycerol, 5% DMSO mixed refrigerated growth trend. Intracellular and extracellular protective agent (glycerol the DMSO glucose) mixed use (10% glycerol 5% DMSO 15% glucose) under the protection of the experimental group MIX1 altar seaweed refrigerated best results, refrigerate the 90d of survival up to 95% and grew well . 4. Salinity and algal cell wall thickness and refrigeration effect. When the salinity of 25 refrigerated algae cell survival rate is higher than other salinity experimental group; frond cell wall thickness affect the preservation effect, the same strain of algae, cell wall thickness than the thin wall cells are more resistant to low temperature survival rate; survival rate differences in different parts of the same strains of algae refrigerated 30d. The experiments also showed that the refrigerated the better its frond contained higher phycobiliprotein total, the survival of the frond growth status and phycobiliprotein content. Altar the laver with large miscellaneous algae (green laver, Enteromorpha, Ulva pertusa, really Gracilaria, Shuiyun) resistance to dry the dew capable of significant differences. Large hybrid algae dry gel (fans downshift continued dry gel, all died of the same below) to 2h, The altar seaweed in the dry gel 3h (has less than 10% moisture content) above only started a small amount of death. The large hybrid algae without the dry exposed refrigerated 5d all died; laver of the altar without dry gel (moisture content of about 70%) under refrigerated conditions, 5d survival rate of over 90%, with the time extension of the refrigerator, the survival rate gradually decreased. Large hybrid algae Concha 0.5h refrigeration the 30d had all death, altar laver (a moisture content of approximately 10% to 15%) survival rate was 100%. Benthic diatoms (the oval algae and Navicula) on somewhat less tolerance of dry gel and refrigerated. Practice production, the altar seaweed refrigerated network second crop farming harvest high quality altar seaweed 418.1 kg / mu (first crop yield of 175.2kg / acre, the second crop of 242.9 kg / mu), without refrigeration altar seaweed harvested a total of 317.5kg / acre harvested a total of 175.0kg / mu (three times, three times harvested 142.5 kg / mu), the production of high-quality seaweed harvested refrigerated network is much higher than the unrefrigerated. In addition, through the refrigerated haitanensis phycobiliproteins, chlorophyll content was significantly higher than the altar seaweed has not been refrigerated.

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CLC: > Agricultural Sciences > Aquaculture, fisheries > Product water transport,preservation,storage,processing,packaging > Aquatic products, storage and storage equipment > Algae storage
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