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The Investigate of Molecular Epidemiology of PCV2 in Anhui

Author: YangShiXing
Tutor: WeiJianZhong；FanWeiXing
School: Anhui Agricultural University
Course: Preventive Veterinary Medicine
Keywords: Porcine circovirus type II Molecular Epidemiology Survey
CLC: S858.28
Type: Master's thesis
Year: 2006
Downloads: 125
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Abstract

Porcine circovirus disease is a viral infectious disease of pigs caused by the the porcine circovirus II (PCV2) of a multiple-system dysfunction. Clinical to newborn piglets congenital tremor and weaned piglets multi-system failure syndrome as the main feature. Post-weaning multisystemic wasting syndrome (postweaning multisystemic wasting syndrome.PMWS) pathogens have now been recognized as porcine circovirus (PCV2) In addition to the trigger PMWS, PCV2 and porcine dermatitis and nephropathy syndrome (porcine Dermatitis and nephropathy syndrome PDNS), newborn piglets congenital brain tremor (Congenital tremors.CT), proliferative and necrotizing pneumonia (proliferative and necrotizing pneumonia.PNP) and pregnant sows reproductive disorders (Reproductive disorders) the occurrence of the disease, it has to The pork industry worldwide has caused huge economic losses, domestic porcine circovirus infection, prevention, treatment, and even disease research is essential for us. This study is based Anhui Province, the geographical distribution and the status of the farming, pig tissue and organ samples collected from the different regions of Anhui epidemiological investigation. 1 according to PCV2 gene sequence has been published in the Genbank, select highly conserved the ORF2 in its internal design a pair of primers (sequences amplified fragment of 630bp) PCR detection of two strains of PCV2 reference strains, can 630bp gene fragment was amplified PCV1, classical swine fever virus, chicken infectious anemia virus were not amplified specific target band. The PCV2 reference strains for different dilution were PCR amplified, and when diluted to 100 times, the i.e. 5fg virus DNA content can still amplify specific fragments. The application of this test method slaughterhouse pigs collected in four regions in in Suzhou, huaining, Fuyang, Hefei spleen, lung, kidney, lymph nodes each 30 and collected in CHONG Shucheng winds, Chaohu Xiongye 3 pigs field incidence of pig spleen, lung, kidney, lymph nodes in each 10, and the results show that: (1) in different parts of the slaughterhouse pigs have porcine circovirus II infection, which, Suzhou, Hefei detection rate of 6.1% Fuyang detection rate of 10%, while the Huaining the detection rate of 23.3%. (2) The survey showed that three different incidence of pig farms: Shucheng Changfeng pig farm, and Chaohu Xiongye Food Co., Ltd. pig farm pigs detection rate of 20%, limited of Hua Jieliang livestock and poultry detection rate of 70%. In order to understand the tissues and organs of infected virus infection status; respectively above has been detected as a positive sample different parts of the detection; detection site, including the spleen, lung, kidney, lymph nodes, etc.. The results showed that the different parts have a positive detection of the target fragment, but different parts of the detection rate of the type. In the 20 samples tested, spleen and lymph node detection rate was 100% detection rate of 80% of the lung kidney detection rate of 40%. Thus indicating that the virus is mainly against immune organs, which may be the reasons for the pig immune function decreases. In order to compare the detection of PCV2 with other domestic and foreign strains kinship Anhui, on the part of the positive samples detected by PCR amplification and sequencing and sequence analysis. Show that the the PCV2 sequence of the PCR products of the four samples in the test sequence in GenBank landing by sequencing more than 90% homology to prove is PCV2. Can be seen from the phylogenetic tree; close phylogenetic relationship between the four positive samples, they are composed of branches and a strain AY484416, composed of a small branch; this branch and DQ195679 and AY556476, AY556473 three strains closer relationship with an American isolate (AY325495), a Fujian strain (AY556474) together form a branch of the far distance.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Livestock, poultry, wildlife diseases > Livestock > Pig
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