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Bowl lotus varieties ' Hongxia sky ' , using RT-PCR method to clone the the LFY genes and AP1 gene fragment . Laid the necessary foundation for the full-length to obtain the LFY genes and AP1 gene in the future as well as understand the lotus flower molecular mechanism and flowering control . The results are as follows : (1 ) the establishment of a lotus samples of total RNA extraction and purification methods . Summed up by comparing the to improved in Trizol, CTAB-LiCl law , improved CTAB-LiCl French RNA extraction method for lotus sample RNA extraction methods - improved CTAB method . And by this method to obtain a more pure , the integrity of the total RNA, can be directly used in the subsequent RT-PCR reaction . (2) the lotus LFY gene cDNA fragment was cloned by RT-PCR . The LFY gene fragment cloned 431bp, encoding 143 amino acids . Sequence analysis showed that the amino acid sequence identity of the deduced amino acid sequence of the fragment with the Hongye quinoa, apples, grapes, tobacco , petunia , chamomile, and other plants were 95% , 95%, 94 % , 94% , 94% , and 93 %. Phylogenetic analysis showed that lotus apple and loquat genetic relationship recently . ( 3) the lotus 2 the AP1 gene cDNA fragment was cloned by RT-PCR , were named NeAP1-1 , and NeAP1 - 2 . NeAP1-1 and NeAP1-2 nucleotides in length of the gene fragment of 216bp, coding for 72 amino acids. Using DNAMAN software , analysis of NeAP1-1 and NeAP1-2 nucleotide and amino acid sequence homology of 89.35% and 88.89% , respectively . Sequence analysis showed that the NeAP1 1 and NeAP1 , 2 of the amino acid sequence with other plants AP1 amino acid sequence homology of more than 62% . Phylogenetic analysis showed that lotus celandine closest genetic relatives . (4 ) NeAP1-1 and NeAP1-2 amino acid sequence conserved domains predicted , the K-box NeAP1 - 1 to 21 amino acids for the MADS-box domain , 53 to 72 amino acids domain , NeAP1 a 22 amino acid MADS-box domain , 45 to 72 amino acids of the K-box domain .
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