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Bayberry (Myrica rubra Seib. Et Zucc.) Yang, Meco (Myricaceae,) Myrica subtropical fruit trees (Myrica spp.), Is one of the fruits of our specialty. In recent years, Xiangshan County, Zhejiang the local carbon Mei resources survey found a high-quality, large fruit, precocious bayberry plant, tentatively named Wu Zi bayberry. In order to clarify the quality of the new strain accumulation characteristics and determined the molecular level with other varieties of genetic differences between the test Wuzi bayberry fruit development process fruit dry weight, fresh weight, sugar, titratable acidity, anthocyanin content were determined to study the dynamics of the Fruit Development sugar metabolism-related enzyme activity; same time, the use of RAPD marker technology, identification of genetic diversity the Wuzi bayberry mother plant and its grafting offspring and other bayberry varieties of Wuzi Bayberry Genetics Identification of production and cultivation of new varieties provide a theoretical basis. The experiments of this thesis the following major findings: 1, the Wuzi bayberry dry, fresh weight with the development and maturation of the fruit continued to grow, the growth rate in the fruit coloring to mature during this period (about two weeks before the fruit is ripe) to peak. 2, Wuzi bayberry fruit sugar sucrose accumulation, sucrose, total sugar 2/3. Fruit sugar, anthocyanin accumulation with fruit development, the accumulation rate of about 2 weeks ago to fruit ripening begins to rise sharply. Bayberry fruit titratable acidity showing low - high - low mode changes, titratable acidity peak about 2 weeks before the fruit is ripe, Since then, with the rapid decline in fruit coloring and maturity. Ripe fruit, the Wuzi bayberry fruit sugar content, anthocyanin content were higher than Dongkui acid content below Dongkui; the The Wuzi bayberry quality is better than Dongkui. 4, the Wuzi waxberry sucrose decomposition activity (invertase activity and SS decomposition activity) changed little, and lower than the carbon plum, charcoal plum sucrose decomposition activity increased with fruit development showed an upward trend; ripe fruit Wuzi Bayberry sucrose synthetase activity (SPS and SS synthesis activity) were higher than carbon Mei, 2 varieties of SPS activity upward trend with the fruit development, but SS synthesis activity peak in the middle of the fruit development. 5, FRK activity was lower than in the mature Wuzi bayberry HXK, and mature carbon plum FRK activity than HXK activity. Biochemical analysis showed that 2-3 weeks before harvest fruit full ripe this time is the formation of a critical period of development and quality of bayberry fruit dry matter accumulation and fruit closely related to water input; 2 bayberry varieties sucrose metabolism activity difference is caused by sugar form the main reason for the different parts of glucose and fructose ratio difference between the two varieties may be FRK fruit HXK activity difference is caused. 6, Xiangshan Wuzi Bayberry new line mother tree, mother tree near the male plants (male plants, male plants), the Wuzi waxberry Grafted (grafting the first generation, second generation, third generation, 4th generation) as test materials, local carbon Mei, the local water the plum and Dongkui for control, using RAPD (RANDOMLY amplified Polymorphic DNA, random amplified polymorphism DNA) technology, on the the 10 Bayberry material genome polymorphism were measured, and based on the results of cluster analysis, analysis of its genetic similarity and genetic distance; more than 150 random primers for PCR amplification of the sample genomic DNA, filter out 47 amplification reproducible bands were clear and legible random polymorphic primers, from the 47 polymorphic primers were amplified 2403 DNA fragment, wherein the the 1195 fragment showed polymorphism. Electrophoresis of PCR amplification products cluster analysis, results show that the Wuzi the bayberry 1-4 generation with the highest similarity of the mother plant, between 0.94-0.98; Wuzi bayberry and East Quebec, charcoal plum, water plum similarity is low, about 0.80. The genetic distance analysis also showed that among the closest in the the Wuzi bayberry mother tree and grafted offspring, the followed by Dongkui, charcoal plum again, and as far as the distance between the water and the plum. 8 In this study, the physiological and biochemical analysis and molecular marker analysis showed that the Wuzi Bayberry is a different from other varieties of bayberry, genetic performance and stability of the new system.
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