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Fine Mapping of Seed Color Gene in the Resynthesized Yellow-seed Barssica Napus

Author: LiXia
Tutor: TuJinXing
School: Huazhong Agricultural University
Course: Crop Genetics and Breeding
Keywords: Synthetic Brassica YELLOW-SEEDED Seed coat color gene AFLP SSR ACGM Molecular markers
CLC: S565.4
Type: Master's thesis
Year: 2009
Downloads: 63
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Abstract


The same genetic background , YELLOW-SEEDED black seed : high oil and protein content , the kind of thin , oily crystal clear , meal cellulose content is low . Therefore , based on the many advantages of the yellow seed traits of Brassica napus yellow seed trait is becoming a hotspot of modern the Rape quality breeding . YELLOW-SEEDED synthetic Brassica , Arabidopsis and Brassica genome sequences of known information for materials, make full use of the two near-isogenic line , YELLOW-SEEDED synthetic Brassica seed coat color gene fine mapping . The main findings are as follows : 1 ) the use of Group Analysis (BSA) with the AFLP technique combined screening seed coat color gene molecular markers linked to synthetic Brassica YELLOW-SEEDED near- isogenic line populations Communists a difference 20 fragments , which EA15MG9 target gene distance 1.2cM. Of Brassica campestris groups received a total of 52 different fragments . 2) of which eight AFLP markers recovery , cloning , sequencing , sequencing results , of which the five differences fragments design specific primers SCAR marker , the results showed that: successfully converted two of which were named for the S159 and S615 S159 from Brassica napus groups AFLP markers EA15MG9 S615 from Brassica campestris L. groups the AFLP markers EA6MG15. 3) the AFLP markers the sequence submitted http://www.brassica-rapa.org/ BLAST analysis , design basis results of SSR primers , 42 differentially labeled in the two groups , and the position of the target gene the ninth linkage groups of the A genome . 4 ) According to the ninth linkage group published molecular marker genetic linkage map collecting , sorting , screening 35 primer pairs , 10 pairs of differentially labeled . 5 ) 25 pairs of primers based on the Arabidopsis information design ACGM Brassica napus groups did not show differences in the five pairs of primers showed differences in the populations of Brassica campestris . While taking advantage of the the Arabidopsis the TT gene design ACGM mark , cabbage groups also received two differentially labeled , verified by one of the groups that were isolated marker .

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CLC: > Agricultural Sciences > Crop > Economic crops > Oil crops > Rapeseed ( Brassica )
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