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Large group sac Cordyceps (Cordyceps ophioglossoides) is China a traditional dilute your medicinal fungi are the Ascomycota ( Ascmycotina ) , meat seat bacteria mesh ( Order Hypocreales ) of ergot Branch ( Clavicipticeae ) , Cordyceps genus (Cordyceps), Ancient medical books , the Pharmacopoeia documented large group capsule Cordyceps improve human immunity, the efficacy of the treatment of vaginal bleeding and irregular menstruation . Continue to damage the wild , the sharp drop in drug source the wild large group capsule Cordyceps endangered , is difficult to manually collected , so the use of modern fermentation technology for large group carried out a large-scale production of medicinal capsule Cordyceps clonal strains is necessary . To train a large group of the subject to the artificial separation capsule Cordyceps strains as pure strains, fermentation process optimization on the basis of the basic fermentation medium , fermentation mycelium medicinal components analysis for the large group of capsule Cordyceps fermentation to enlarge laid the foundation and development of new drugs . ( Sucrose 66g / l , yeast extract 10g / L , silkworm chrysalis hydrolyzate 3.0 % ( v / v ) in the fermentation medium on the basis of the established of the over MgSO 4 · 7H O 0.4g / l, anhydrous KH 2 PO 4 0.4g / l) , fixed speed of 150rpm and the initial aeration rate (1:0.6 vvm), with L 9 ( 3 4 < / sup >) orthogonal test to detect indicators hyphae , on a 15 - liter fermentor fermentation process main factors optimization . The results show that the better fermentation conditions : 5% of the inoculum size , fermentation temperature 23 ° C , pH 5.5 , 40h after adjust ventilation to 1:1 vvm . Above the better fermentation process conditions , a large group of capsule Cordyceps production of up to 9.5g / L ( dry weight ) , nearly double than that before optimization . The large group of fermentation capsule Cordyceps medicinal components analysis , the measurement method , the results show that its main active ingredients in the above the better fermentation process conditions were Cordyceps polysaccharide 14.06mg / g, Cordyceps acid 58.89mg / g , total protein 74.79mg / g , adenosine 2.17mg / g.
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