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The Frequencies of Autotetraploid Rice with Polyembryonic Seedling and Its Reproductive Characteristics

Author: HuXiuMing
Tutor: HuangQunCe
School: Zhengzhou University
Course: Biophysical
Keywords: Autotetraploid rice Twin seedlings Tissue culture system Reproductive and developmental characteristics
CLC: S511
Type: Master's thesis
Year: 2007
Downloads: 114
Quote: 1
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Abstract

In the field of study of the genetic improvement of rice researchers are currently facing two major problems to be explored, namely how through modern biotechnology in the existing level of production more efficient use of the of rice heterosis effect mining its yield potential and how by simplifying breeding program Fixed rice heterosis effect. The results of previous studies have confirmed that polyploidy genome level to explore the potential value of autotetraploid rice genetic improvement of rice will find a new direction. Ion beam implantation technology \the the lines relative stability of its characteristics. On this basis, the study compared the performance characteristics of mutant staining techniques, ovary overall transparent technology and laser scanning confocal microscopy twin-seedling rice autotetraploid observational study of developmental characteristics of male and female gametes (male and female gametophyte) system designed for the accumulation of ion beam bio-technology in the genetic improvement of rice research data. The preliminary results of this study include the following three aspects: 1. Mutation screening and improved body characteristics of research objectives, to establish a the autotetraploid twin-seedling rice tissue culture system, which screened seed rate is relatively high material. In the experiment, by the Comparative Study of tissue culture mature embryos tested materials callus induction rate and quality to a large extent depend on the hormone content in the induction medium. In the differentiation medium plays a very important role in the induction of the relative proportions of various hormones and trace element content of Cu is for the regenerated plants. Autotetraploid twin-seedling rice plant regeneration groups screened twin seedlings seed setting rate a plant height of plants, seed setting rate up to 71.8%. 2. For research purposes, the expression of the mutant was identified by the appearance of features and characteristics of twin seedlings conditions, temperature and other factors on the incidence of twin seedlings. The results show that, with the propulsion of selfing twin seedlings maintain genetic stability characteristics. In groups of experimental material, the frequency of polyembryonic seedlings showed some differences due to the different types of materials or germination of different temperature conditions and the presence or absence of rice glume. Germination temperature is 30 ℃ ASDOR05-01 and ASDOR05-02 twin seedlings frequency than higher by 6.8% and 2.5%, respectively. The seed went glume treatment can make the the twin seedlings frequency increased by 36.0% -44.1%. Twin seedlings mutant strains \The twin seedlings homozygous lines in the same group Tony bit including the the incomplete twin seedlings and fully double-seedling two types. Incomplete twin seedlings can be further divided into single hypocotyl single The radicle the twin seedlings and single radicle different hypocotyl two types of twin seedlings. Completely twin seedlings can be further divided into two types of normal twin seedlings and abnormal twin seedlings (size seedlings). The-seedling material, the single radicle different hypocotyl share twin seedlings ratio is relatively large. 3. In order to study the developmental characteristics of the mutant male and female gametes (body), this study using fluorescent staining technique, the ovary overall transparent technology and laser scanning confocal microscopy of twin-seedling rice autotetraploid \The original parent IR36-4X reproductive development process carried out detailed observations, and identified the source of its multi-embryo embryo. The observed results indicate that the former male gametophyte development process to produce a higher frequency Abortive pollen grains, pollen grains sterile type frequencies up to 47.3% (typical abortive type, round-Abortive and stained abortion type pollen grains were 3.8%, 5.4% and 38.1%, respectively). \The abortive state the main reason microspore trends in the development of late pollen grains. \Found in the female gametophyte development characteristics observed identification process, all test materials were there the female gametophyte abortion and embryo sac abnormalities phenomenon, but abortion frequency and abnormal embryo sac development, the number of different material varies. The mutant frequency of abnormal embryo sac (34.7%) is significantly higher than that of the original parents (24.5%). \

The Frequencies of Autotetraploid Rice with Polyembryonic Seedling and Its Reproductive Characteristics

Abstract

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