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Study on High Quality of Penniseetum by Morphological and RAPD Marker

Author: ZhuJun
Tutor: YangXiaoHong；ChenPing
School: Southwestern University
Course: Botany
Keywords: Pennisetum Morphology RAPD Mark Genetic Diversity
CLC: S543.9
Type: Master's thesis
Year: 2007
Downloads: 121
Quote: 1
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Abstract

Pennisetum (Penniseetum) is an important forage resources are widely distributed throughout the world, the majority originating in Africa and around the world more than 140 kinds. Pennisetum quality forage resources are relatively tall plants, higher yield and nutritional quality of its many varieties, good palatability. Not only that, With further research, it is in the other aspects of the application is also gradually people are familiar with and take advantage of the introduction and breeding of quality Pennisetum becomes more and more important. Pennisetum repeatedly intraspecific and interspecific hybridization and breeding pro-utilization is relatively concentrated, the morphological differences between the varieties are getting smaller and smaller, resulting in morphological identification difficult of Pennisetum excellent grass species, the The reason is the variety between genetic variations. In view of this, this paper Pennisetum case as the research object, select Zhongkai Agrotechnical College planted 11 different varieties of high-quality Pennisetum establishment of RAPD reaction system and response procedures for Pennisetum and by morphological and RAPD molecular markers comparative study of Pennisetum varieties identified after analysis of variance and cluster analysis, to explore the genetic variability in the relationship between the molecular level and morphological phenotype. The main findings are as follows: 11 Pennisetum varieties of cutting seedling growth after 18 months, the measuring field plants natural height, absolute height, internode length, stem height, section Minister axis and short-axis section number, leaf length, leaf width nine morphological indicators and morphological differences analysis. The results show that: the coefficient of variation of 11 grasses nine morphological indicators, the minimum is 28%, the largest can reach 43%. Cluster analysis shows that these morphological index of 11 Pennisetum varieties, 11 the Pennisetum species can be divided into two categories, the morphological characteristics of a class Pennisetum relatively tall plant morphology: Another wolf tail grass plants, leaf length and leaf width is relatively small. By orthogonal experiments and single-factor repeated optimization, a the best PCR reaction system and procedures. The reaction system: Twenty (20) μL of total volume. Between the concentration of each component: 1 × buffer, Mg ^{2 2mmol / L dNTPs 150 μmol / L, and Taq polymerase solution consisting of 0.75 u, primer to 0.5μmol / L, the stencil DNA25ng. After mixing the ingredients added, double distilled water to make not sufficient volume after sterilization. Amplification program: 94 ° C denaturation at 2min; 94 ° C denaturation 20s, 37 ° C renaturation 30s, 72 ° C extension 90s, set for 40 cycles; final save in 72 ℃ for 10min, 4 ° C. 50 random primers were screened, and a total of eight for Pennisetum RAPD analysis of random primers. Amplified total bar with 82 polymorphic bands 60 polymorphic loci was 73.2%, the percentage of polymorphic loci between 57.1% -85.7%. Average per primer amplified 10 with a maximum of 14, at least there are seven. Pennisetum morphological markers and RAPD map measured analysis of clustering, the obtained genetic tree. RAPD analysis the dendrogram and morphological marker analysis dendrogram result there are some differences between the morphological markers and RAPD markers. Morphological analysis of some samples deviate from where varieties group, and RAPD markers classification results are not totally consistent. The reasons for this situation are many, on the one hand, morphological indicators by the environmental impact, even similar species, their best growth environment may also be completely inconsistent; the other hand Napiergrass breeding parental relatively The more concentrated the morphological differences between varieties are also getting smaller and smaller, single morphology has been difficult to discern. Of course caused morphological analysis and molecular level RAPD analysis of different reasons are many and varied, and these are subject to further research and feasibility studies.}

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