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Today's society a lot of chemical pesticides used in agricultural production, pesticide residues form a huge threat to human health and ecological environment. Now almost all of the environment, such as soil, plants, water animals can be found pesticide residues, especially the particularly serious harm to the water environment and aquatic. More common in a wide range of chemical pesticides, organophosphorus pesticide pollution caused harm, often poisoning incident, organophosphorus pesticide poisoning, muscle, nerve, brain, heart and circulatory system are serious injuries. Glufosinate C 5 H 15 N 2 OP 4 Ammonium-(3-amino-3-carboxy propyl )-methl phosplunate trade name hundred test up to (Basta) system the organophosphate herbicide developed by BASF, can control grass and broadleaf weeds. Been widely used due to effective weed control. With the in-depth knowledge of the toxicity and environmental risks of glufosinate residue to the ecological environment safety and human health, and an urgent need to develop a more efficient and sensitive detection methods. At present, most of the detection of pesticide residues are using chromatographic techniques (such as gas chromatography and liquid chromatography), but these traditional techniques are expensive, time-consuming, can not detect large quantities of samples at the same time, while immunoassay (ELISA) as a fast and low-cost detection method developed rapidly in the past ten years, together with chromatographic techniques become pillars of technology for the detection of pesticide residues. Pesticide immunoassay key is to prepare highly specific antibodies, in order to improve the specificity and sensitivity of detection. The advent of hybridoma monoclonal antibody technology to provide the possibility abroad has been produced by a variety of pesticides monoclonal antibody (Monoclone antibodies, McAb) and applied to the analysis of pesticide residues in the immune. In recent years, the domestic application of monoclonal antibodies in development in the detection of pesticide residues, but at home and abroad have not yet found glufosinate residue of the enzyme-linked immunosorbent assay (ELISA). Research. Synthesis and characterization of glufosinate hapten hapten and carrier protein coupled with the identification and preparation of a high titer glufosinate monoclonal antibody. Aims to further establish and glufosinate ELISA analysis method applied to the analysis of pesticide residues. Glufosinate a molecular weight of less than 1,000, can not cause an immune response, so we use the cross-linking agent will connect to the glufosinate ovalbumin (OVA), using a multi-point injection mice immunized subcutaneously results obtained high titer polyclonal antibody. Preparation and identification of monoclonal antibodies specific glufosinate and glufosinate detection.
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