Dissertation > Excellent graduate degree dissertation topics show

Studies on the Effect and Mechanisms of Tamoxifen on Endometrium

Author: ZhangMei
Tutor: YuanBenLi
School: PLA Military Academy of Medical Sciences
Course: Pathology and Pathophysiology
Keywords: Tamoxifen endometrium proliferation apoptosis estrogen progesterone ER PR
CLC: R96
Type: Master's thesis
Year: 2007
Downloads: 116
Quote: 0
Read: Download Dissertation

Abstract


Objective: The study was conducted to investigate the effects of tamoxifen（TAM）on rat’s endometria of bilateral ovariectomy and sham surgery, and on the cell lines of MCF-7、ISK and Hela. It maybe provide theory to elucidate the possible mechanism of TAM induced endometrium pathogenesis, and program to prevent and therapy pathological change of endometrium.Methods: Some methods, Light microscope and transmission electron microscopeobserved rat endometrium, immunohistochemistry、tunnel and Westen blot determined affection TAM on endometrium, were used in the study in vivo. The other methods, MTT、immunofluorescence and flow cytometry, were also used to examine affection of TAM on MCF-7 cell、ISK cell and Hela cell in vitro.Results: 1、Some toxicities were found for rat in dose of TAM 50 mg·kg-1 ig for 28days continuesly, including lose body weight、lipsotrichia and pathological changes of endumetria. 2、In ovariectomized rat group, TAM increased uterus weight and uterus to body weight ratio, and increased serum estrogen level. In sham group, TAM decreased uterus weight and uterus to body weight ratio, and decreased serum estrogen level. At two groups, TAM reduced the expression of uterus Erβand serum progesterone level, and elevated the expression of uterus PR. 3、After TAM administration, a slight indication showed that the positive cells of PCNA was fewer in sham group than control and that was more in ovariectomized group. Apoptosis cell significantly decreased compared with control group respectively. 4、TAM was able to inhibit cell proliferation for MCF-7and facilitate proliferation for ISK cell and Hela cell, but at the letter cells TAM could inhibit proliferation when lower than a micromolecular concentration. TAM could induce apoptosis of MCF-7 cell and slight inhibit apoptosis of ISK cell. TAM could depress the expression of bcl-2 and increase the expression of bax in the MCF-7. The result was contrary in the ISK cell and Hela cell.Conclusions: 1、TAM was able to induce the pathological changes of ratendometrium and some toxicological reaction after 50 mg·kg-1 ig 28 days continuesly. 2、TAM expressed twofold effects in deffrent serum estrogen level. TAM had the estrogen-like effect on endometrium in low estrogen and antiestrogen effect in high estrogen. 3、Main action of TAM was not stimulated endometrium proliferation, but inhibit endometrium apoptosis. 4、TAM had different effects on tumor cells of MCF-7、ISK and Hela. TAM influence proliferation and apoptosis by change oncogene and anti-oncogene.

Related Dissertations

  1. Expression of Prolyl Isomerase Pin1 in Osteosarcoma and the Effect of Regulation on Cell Cycle,R738.1
  2. The Study on Cryopreservation and Mechanism of Freezing Injury on the Spermatozoa of Coelomactra Antiquate,S968.3
  3. The Anti-tumor Effect of CADPE and Inducing Apoptosis in Human Gastric Cancer Cells,R735.2
  4. Cloth and β- elemene combined administration of anti-tumor effect and mechanism of celecoxib,R96
  5. TRAIL in the regulation of tumor invasion CD4 ~ CD25 ~ Treg,R730.2
  6. Study on the Separation and Purification Way of Arctiin from Fructus Arctii and the Effect of Arctiin on the Growth of Mice C2C12 Cells,S853.7
  7. The Study of Lead Exposure on Cyprinus Carpio Ovary Epithelial Cells,X174
  8. Effects of Diclazuril on G3PDH in Second-generation Merozoites of Eimeria Tenella,S858.31
  9. Effects of Kisspeptin-10 on Progesterone Secretion of Chicken Ovary Granulosa Cells and Mechanism Research,S831
  10. The Effects and Preliminary Study of PCV2 on Ca2+ Signal in Lymphocytes of Piglets,S858.28
  11. Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Sexual Maturity Mice,S852.2
  12. Expression of β-Catenin in Pig’s Ovary and the Effect of β-Catenin on Porcine Granulosa Cells Apotosis and Steroidogenesis Related Enzyme,S828
  13. Proliferation Characteristics of Type 2 Porcine Circovirus Strains and Antivirus Activity of Iprs and Aps on PCV2 in Vitro,S858.28
  14. Induced Combination Apoptosis by FB1 and AFB1 in Vero Cell,S856.9
  15. The Apoptosis Mechanism Induced by Aflatoxin B1 and Deoxynivalenol in Primary Hepatocyte of Cyprinus Carpio,S856.9
  16. Effects of BMPR-IB Gene Silencing by Small Interfering RNA on Apoptosis of Porcine Pollicular Granulosa Cells and Ecpression of BMP Pathy-Way-Ralted Genes,S828
  17. The Effects of Don on Proliferation, Differentiation, and Apoptosis of Chondrocytes in Chicken,S858.31
  18. The Effect of Foxol on Apoptosis of Mouse Granule Cells,S865.13
  19. Protection Effects of Pu-er Green Tea Water Extraction Again Trans Fatty Acid,R285.5
  20. Study of Melittin on Tumor Inhibition and Part of Mechanism of Human Hepatocellular Carcinoma HepG-2 Xenograft in Nude Mice,R735.7
  21. The Protective Effect of Rat Myocardial Ischemia/reperfusion Injury Following Bone Marrow Mesenchymal Stem Cell Pretransplantation for 1 Week,R542.22

CLC: > Medicine, health > Pharmacy > Pharmacology
© 2012 www.DissertationTopic.Net  Mobile